Western Blot (WB)
(Western blot analysis of ORAI3 in A20 cell lysate with ORAI3 antibody at (A) 2 and (B) 4 μg/mL.)
Rabbit anti-Human, Mouse ORAI3 Polyclonal Antibody | anti-ORAI3 antibody
ORAI3 Antibody
Gene Names
ORAI3; TMEM142C
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunocytochemistry, Immunofluorescence
Purity
ORAI3 Antibody is affinity chromatography purified via peptide column.
Synonyms
ORAI3; Polyclonal Antibody; ORAI3 Antibody; TMEM142C; Protein orai-3; Transmembrane protein 142C; ORAI calcium release-activated calcium modulator 3; anti-ORAI3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
ORAI3 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid
Concentration
1 mg/mL (varies by lot)
Sequence Length
295
Applicable Applications for anti-ORAI3 antibody
ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ORAI3 antibody can be used for detection of ORAI3 by Western blot at 2 - 4 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 10 mug/mL.
Conjugate
Unconjugated
Immunogen
ORAI3 antibody was raised against a 19 amino acid synthetic peptide from near the carboxy terminus of human ORAI3.
Buffer
ORAI3 Antibody is supplied in PBS containing 0.02% sodium azide.
Preparation and Storage
ORAI3 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(Western blot analysis of ORAI3 in A20 cell lysate with ORAI3 antibody at (A) 2 and (B) 4 μg/mL.)
Western Blot (WB)
(Western blot analysis of ORAI3 in A20 cell lysate with ORAI3 antibody at (A) 2 and (B) 4 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of ORAI3 in A20 cells with ORAI3 antibody at 5 μg/mL.)
Immunocytochemistry (IHC)
(Immunocytochemistry of ORAI3 in A20 cells with ORAI3 antibody at 5 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ORAI3 in A20 cells with ORAI3 antibody at 10 μg/mL.)
Immunofluorescence (IF)
(Immunofluorescence of ORAI3 in A20 cells with ORAI3 antibody at 10 μg/mL.)
Related Product Information for anti-ORAI3 antibody
ORAI3 Antibody: Antigen stimulation of immune cells triggers Ca++ entry through Ca++ release-activated Ca++ (CRAC) channels. ORAI3 is one of two mammalian homologs to ORAI1, a recently identified four-transmembrane spanning protein that is an essential component of CRAC. All three homologs have been shown to function as Ca++ plasma membrane channels gated through interactions with STIM1, the store-activated endoplasmic reticulum Ca++ sensor. However, ORAI3 channels failed to produce detectable Ca++ selective currents in cells co-transfected with ORAI3 and STIM1, indicating that ORAI3 channels undergo a lesser degree of depotentiation than ORAI1 or ORAI2. Na+ currents through ORAI1, 2 and 3 channels were equally inhibited by extracellular Ca++, indicating that each have similar affinities for Ca++ within the selectivity filter. This antibody is predicted to have no cross-reactivity to ORAI1 or ORAI2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
31,499 Da
NCBI Official Full Name
Protein orai-3
NCBI Official Synonym Full Names
ORAI calcium release-activated calcium modulator 3
NCBI Official Symbol
ORAI3
NCBI Official Synonym Symbols
TMEM142C
NCBI Protein Information
protein orai-3; transmembrane protein 142C
UniProt Protein Name
Protein orai-3
Protein Family
UniProt Gene Name
ORAI3
UniProt Synonym Gene Names
TMEM142C
UniProt Entry Name
ORAI3_HUMAN
Uniprot Description
TMEM142C: Key regulator or component of store-operated Ca(2+) channel and transcription factor NFAT nuclear import. Belongs to the Orai family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: integral to membrane
Molecular Function: protein binding
Research Articles on ORAI3
Similar Products
Product Notes
The ORAI3 orai3 (Catalog #AAA151480) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ORAI3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ORAI3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF). ORAI3 antibody can be used for detection of ORAI3 by Western blot at 2 - 4 mug/mL. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 10 mug/mL. Researchers should empirically determine the suitability of the ORAI3 orai3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ORAI3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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