Mouse anti-Human Geminin Monoclonal Antibody | anti-GMNN antibody

Geminin

Cat. Num. AAA604158

• Gene Names: GMNN; Gem; RP3-369A17.3
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Geminin; Monoclonal Antibody; Anti -Geminin; anti-GMNN antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6H3
• Specificity: Recognizes human Geminin.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.

• Applicable Applications for anti-GMNN antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:250-1:500
• Immunogen: Recombinant human Geminin (1-209 aa) purified from E. coli
• Hybridoma: Mouse SP2/O myeloma cells with spleen cells from BALB/c mice.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(The 293T cell lysates(50ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with MBS604158 (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Related Product Information for anti-GMNN antibody

Geminin is a 25kD protein, which inhibits DNA replication and is degraded during the mitotic phase of the cell cycle. Geminin controls replication by binding to the licensing factor Cdt1, and is involved in neural differentiation. In addition, geminin directly interacts with Six3 and Hox homeodomain proteins during embryogenesis and inhibits their functions.

Product Categories/Family for anti-GMNN antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 7705682
• NCBI GeneID: 51053
• NCBI Accession #: NP_056979.1
• NCBI GenBank Nucleotide #: NM_015895.3
• UniProt Accession #: O75496; Q9H1Z1; B3KMM8; Q53GD9
• Molecular Weight: 23,565 Da
• NCBI Official Full Name: geminin
• NCBI Official Synonym Full Names: geminin, DNA replication inhibitor
• NCBI Official Symbol: GMNN
• NCBI Official Synonym Symbols: Gem; RP3-369A17.3
• NCBI Protein Information: geminin; OTTHUMP00000016095; OTTHUMP00000016096; OTTHUMP00000016097
• UniProt Protein Name: Geminin
• Protein Family: Geminin
• UniProt Gene Name: GMNN
• UniProt Entry Name: GEMI_HUMAN

NCBI Description

This gene encodes a protein that plays a critical role in cell cycle regulation. The encoded protein inhibits DNA replication by binding to DNA replication factor Cdt1, preventing the incorporation of minichromosome maintenance proteins into the pre-replication complex. The encoded protein is expressed during the S and G2 phases of the cell cycle and is degraded by the anaphase-promoting complex during the metaphase-anaphase transition. Increased expression of this gene may play a role in several malignancies including colon, rectal and breast cancer. Alternatively spliced transcript variants have been observed for this gene, and two pseudogenes of this gene are located on the short arm of chromosome 16. [provided by RefSeq, Oct 2011]

Uniprot Description

Geminin: Inhibits DNA replication by preventing the incorporation of MCM complex into pre-replication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle. Homotetramer. Interacts with CDT1; inhibits binding of the MCM complex to origins of replication. Interacts with IDAS; targets GMNN to the nucleus, prevents GMNN interaction with CDT1 and competes with IDAS homodimerization. Belongs to the geminin family.

Protein type: Cell cycle regulation; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 6p22.3

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: protein binding; histone deacetylase binding; transcription corepressor activity

Biological Process: organ morphogenesis; negative regulation of DNA replication; protein complex assembly; mitotic cell cycle; negative regulation of transcription, DNA-dependent; negative regulation of cell cycle