Mouse anti-Human Synuclein, alpha, aa61-95 Monoclonal Antibody | anti-SNCA antibody
Synuclein, alpha, aa61-95 (SNCA, 127750, P37840, MGC110988, NACP, PARK1, PARK4, non-A beta Component of AD Amyloid, NACP, Parkinson Disease Familial 1, PD1)
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Dilution: ELISA: 1:1000-1:2000
Western Blot: 1:500-1:2000
Western Blot (WB)
(The recombinant human alpha-synuclein domains were resolved by SDS-PAGE, transferred to PVDF membrane and probed with MBS6006217 (1:1000). Proteind were visualized using IgG (HRP) goat anti-mouse secondary antibody and an ECL detection systemLane 1: Recombinant human α-synuclein (1-60aa)Lane 2: Recombinant human α-synuclein (1-95aa)Lane 3: Recombinant human α-synuclein (61-140aa)Lane 4: Recombinant human α-synuclein (96-140aa))
Western Blot (WB)
(The recombinant human alpha-synuclein domains were resolved by SDS-PAGE, transferred to PVDF membrane and probed with MBS6006217 (1:1000). Proteind were visualized using IgG (HRP) goat anti-mouse secondary antibody and an ECL detection systemLane 1: Recombinant human α-synuclein proteinLane 2:Recombinant human β-synuclein proteinLane 3: Recombinant human γ-synuclein protein)
Immunofluoresence/Immunocytochemistry (IF/ICC)
(Immunocytochemistry/Immunofluoresence α-synuclein in HeLa cells. The cells were stained with MBS6006217 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the nucleus (blue).)
NCBI and Uniprot Product Information
NCBI Description
Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene. [provided by RefSeq, Mar 2009]
Uniprot Description
SNCA: a member of the synuclein family. Abundantly expressed in the brain. Inhibits phospholipase D2 selectively. May integrate presynaptic signaling and membrane trafficking. Implicated in the pathogenesis of Parkinson's disease. A major component of amyloid plaques in the brains of patients with Alzheimer's disease. Two alternatively spliced isoforms transcripts have been identified.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 4q21
Cellular Component: Golgi apparatus; nuclear outer membrane; mitochondrion; rough endoplasmic reticulum; lysosome; fibril; extracellular region; terminal button; cell cortex; inclusion body; mitochondrial respiratory chain complex I; cytosol; actin cytoskeleton; synaptic vesicle; platelet alpha granule membrane; growth cone; axon; perinuclear region of cytoplasm; cytoplasm; plasma membrane; ribosome; cell junction; nucleus
Molecular Function: identical protein binding; protein domain specific binding; histone binding; zinc ion binding; kinesin binding; ferrous iron binding; microtubule binding; caspase inhibitor activity; magnesium ion binding; beta-tubulin binding; phosphoprotein binding; protein N-terminus binding; calcium ion binding; Hsp70 protein binding; oxidoreductase activity; dynein binding; protein binding; phospholipase binding; copper ion binding; phospholipid binding; tau protein binding; fatty acid binding; alpha-tubulin binding
Biological Process: regulation of long-term neuronal synaptic plasticity; negative regulation of serotonin uptake; regulation of acyl-CoA biosynthetic process; positive regulation of apoptosis; adult locomotory behavior; mitochondrial membrane organization and biogenesis; negative regulation of norepinephrine uptake; microglial cell activation; positive regulation of endocytosis; response to lipopolysaccharide; dopamine biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of monooxygenase activity; negative regulation of caspase activity; fatty acid metabolic process; positive regulation of neurotransmitter secretion; regulation of dopamine secretion; negative regulation of dopamine uptake; negative regulation of histone acetylation; calcium ion homeostasis; negative regulation of exocytosis; response to magnesium ion; negative regulation of protein amino acid phosphorylation; behavioral response to cocaine; phospholipid metabolic process; receptor internalization; synapse organization and biogenesis; fibril organization and biogenesis; dopamine uptake; response to iron(II) ion; negative regulation of neuron apoptosis; positive regulation of receptor recycling; aging; caspase activation; response to drug; neutral lipid metabolic process; protein destabilization; regulation of glutamate secretion; negative regulation of microtubule polymerization; regulation of macrophage activation; positive regulation of peptidyl-serine phosphorylation; negative regulation of dopamine metabolic process; organelle ATP synthesis coupled electron transport; regulation of locomotion; synaptic vesicle endocytosis; positive regulation of release of sequestered calcium ion into cytosol; regulation of excitatory postsynaptic membrane potential; negative regulation of transporter activity; negative regulation of apoptosis
Disease: Parkinson Disease 4, Autosomal Dominant; Dementia, Lewy Body; Parkinson Disease 1, Autosomal Dominant
