Rabbit anti-Human RNF6 Polyclonal Antibody | anti-RNF6 antibody

RNF6, CT (RNF6, SPG2, E3 ubiquitin-protein ligase RNF6) (Azide free) (HRP)

Cat. Num. AAA6342966

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RNF6; Polyclonal Antibody; CT (RNF6; SPG2; E3 ubiquitin-protein ligase RNF6) (Azide free) (HRP); E3 ubiquitin-protein ligase RNF6; anti-RNF6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 685

• Applicable Applications for anti-RNF6 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: RNF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 528-558 amino acids from the C-terminal region of human RNF6.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RNF6 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the RNF6 antibody detected the RNF6 protein (arrow).)

Related Product Information for anti-RNF6 antibody

The protein encoded by this gene contains a RING-H2 finger motif. Deletions and mutations in this gene were detected in esophageal squamous cell carcinoma (ESCC), suggesting that this protein may be a potential tumor suppressor. Studies of the mouse counterpart suggested a role of this protein in the transcription regulation that controls germinal differentiation. Multiple alternatively spliced transcript variants encoding the same protein are observed.

Product Categories/Family for anti-RNF6 antibody

Antibodies; Ubiquitin Ligases

References

Xu, K., et al. Cancer Cell 15(4):270-282(2009). Zhu, L., et al. Sci. China, C, Life Sci. 51(4):302-307(2008). Tursun, B., et al. Genes Dev. 19(19):2307-2319(2005). Lo, H.S., et al. Cancer Res. 62(15):4191-4193(2002). Lopez, P., et al. Mol. Cell. Biol. 22(10):3488-3496(2002).

NCBI and Uniprot Product Information

• NCBI GI #: 34305295
• NCBI GeneID: 6049
• NCBI Accession #: NP_898865.1
• NCBI GenBank Nucleotide #: NP_898865.1
• UniProt Accession #: Q9Y252
• NCBI Official Full Name: E3 ubiquitin-protein ligase RNF6 isoform 1
• NCBI Official Synonym Full Names: ring finger protein 6
• NCBI Official Symbol: RNF6
• NCBI Protein Information: E3 ubiquitin-protein ligase RNF6
• UniProt Protein Name: E3 ubiquitin-protein ligase RNF6
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: RNF6
• UniProt Synonym Gene Names: SPG2
• UniProt Entry Name: RNF6_HUMAN

NCBI Description

The protein encoded by this gene contains a RING-H2 finger motif. Deletions and mutations in this gene were detected in esophageal squamous cell carcinoma (ESCC), suggesting that this protein may be a potential tumor suppressor. Studies of the mouse counterpart suggested a role of this protein in the transcription regulation that controls germinal differentiation. Multiple alternatively spliced transcript variants encoding the same protein are observed. [provided by RefSeq, Jul 2008]