Testing Data
(Hella Cells were treated with 10 mug BrdU for 1 hour (B) or left untreated (A). Cells were stained with rabbit anti-BrdU antibody at a dilution of 1/500. As a secondary antibody, sheep anti rabbit IgG DyLight 549 conjugated antibody was used at a 1/50 dilution. PureBlu DAPI (1351303) was used as nuclear counterstain.)
Rabbit BrdU Polyclonal Antibody | anti-BrdU antibody
Rabbit Anti BrdU
Applications
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Purity
Purified IgG was prepared from whole serum by affinity chromatography.
Synonyms
BrdU; Polyclonal Antibody; Rabbit Anti BrdU; BrdU antibody; anti-BrdU antibody
Host
Rabbit
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified IgG was prepared from whole serum by affinity chromatography.
Form/Format
Liquid; Phosphate Buffered Saline
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for anti-BrdU antibody
Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin
Immunogen
Bromodeoxyuridine conjugated to KLH
Preservative Stabilizers
0.09% Sodium Azide (NaN3)
Preparation and Storage
Store at -20 degree C only.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Testing Data
(Hella Cells were treated with 10 mug BrdU for 1 hour (B) or left untreated (A). Cells were stained with rabbit anti-BrdU antibody at a dilution of 1/500. As a secondary antibody, sheep anti rabbit IgG DyLight 549 conjugated antibody was used at a 1/50 dilution. PureBlu DAPI (1351303) was used as nuclear counterstain.)
Testing Data
(Hella Cells were treated with 10 mug BrdU for 1 hour (B) or left untreated (A). Cells were stained with rabbit anti-BrdU antibody at a dilution of 1/500. As a secondary antibody, sheep anti rabbit IgG DyLight 549 conjugated antibody was used at a 1/50 dilution. PureBlu DAPI (1351303) was used as nuclear counterstain.)
Testing Data
(BrdU-labeled led human lymphoma cells were stained with rabbit anti-BrdU antibody at a 1/50 dilution (A) and a 1/100 dilution (B). Sheep anti Rabbit IgG DyLight 488 conjugated antibody was used as the secondary detection reagent at a 1/50 dilution. READIDROP Propidium Iodide (1351101) was used to stain total DNA.)
Testing Data
(BrdU-labeled led human lymphoma cells were stained with rabbit anti-BrdU antibody at a 1/50 dilution (A) and a 1/100 dilution (B). Sheep anti Rabbit IgG DyLight 488 conjugated antibody was used as the secondary detection reagent at a 1/50 dilution. READIDROP Propidium Iodide (1351101) was used to stain total DNA.)
Testing Data
(Jurkat cells were treated with 10 muM BrdU (A), CldU (B), IdU (C), EdU (D), Thymidine (E) or left untreated (F). Cells were stained with 1 mug/ml Rabbit anti BrdU antibody for 1 hour at room temperature. As a secondary antibody, Sheep anti Rabbit IgG DyLight488 conjugated antibody was used at a 1/100 dilution. Propidium Iodide was used to stain total DNA.)
Testing Data
(Jurkat cells were treated with 10 muM BrdU (A), CldU (B), IdU (C), EdU (D), Thymidine (E) or left untreated (F). Cells were stained with 1 mug/ml Rabbit anti BrdU antibody for 1 hour at room temperature. As a secondary antibody, Sheep anti Rabbit IgG DyLight488 conjugated antibody was used at a 1/100 dilution. Propidium Iodide was used to stain total DNA.)
Related Product Information for anti-BrdU antibody
Rabbit anti BrdU antibody recognizes bromodeoxyuridine (known as BrdU or BrdUrd). BrdU is a synthetic thymidine analog, which when added to cell culture media can be incorporated into the DNA of replicating cells as a substitute for thymidine. This characteristic combined with the ease of detecting incorporated BrdU with the help of anti-BrdU antibodies has resulted in establishing BrdU as a key tool for the visualization of cells in the S-Phase of the cell cycle.
BrdU is also commonly used in vivo for monitoring cell proliferation in model systems; BrdU can be administered via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).
The cross reactivity of Rabbit anti BrdU antibody to other thymidine analogs, such as 5?-chloro-2?-deoxyuridine (CldU), 5?-iodo-2?-deoxyuridine (IdU) and 2?-deoxy-5-ethynyluridine (EdU) has not been analyzed. However, the antibody does not appear to detect endogenous thymidine.
BrdU is also commonly used in vivo for monitoring cell proliferation in model systems; BrdU can be administered via intraperitoneal injection, subcutaneous osmotic pump implants (Tesfaiqzi et al. 2004) or in drinking water (Moser et al. 2004).
The cross reactivity of Rabbit anti BrdU antibody to other thymidine analogs, such as 5?-chloro-2?-deoxyuridine (CldU), 5?-iodo-2?-deoxyuridine (IdU) and 2?-deoxy-5-ethynyluridine (EdU) has not been analyzed. However, the antibody does not appear to detect endogenous thymidine.
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Product Notes
The BrdU (Catalog #AAA225570) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BrdU can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the BrdU for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BrdU, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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