Testing Data
(Immunolocalization of BMP4 (red) in the lateral ventricle area of P7 mouse CNS. The blue stain is Hoechst nuclear counter stain. Photo courtesy of Dr. Jason Emsley, Harvard Medical School, Boston, MA.)
Mouse Bone Morphogenetic Protein 4 Monoclonal Antibody
Bone Morphogenetic Protein 4 (BMP4, DVR4)
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunocytochemistry
Purity
PurifiedPurified
Synonyms
Bone Morphogenetic Protein 4; Monoclonal Antibody; Bone Morphogenetic Protein 4 (BMP4; DVR4); Anti -Bone Morphogenetic Protein 4 (BMP4; anti-Bone Morphogenetic Protein 4 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
3h2.3
Specificity
Recognizes mouse BMP4. Recognizes human and mouse BMP4 in both reduced and non-reduced conditions. Does not crossreact with human BMP2 or human TGF-b1.
Purity/Purification
Purified
Purified
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Applicable Applications for anti-Bone Morphogenetic Protein 4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
Suitable for use in ELISA, Western Blot and Immunocytochemistry.
Dilution: Western Blot (Reduced and Non-reduced): Using osteoinductive cells, i.e. BFP and Saos-2.
Immunocytochemistry: Using osteoinductive cells, i.e. BFP and Saos-2.
Dilution: Western Blot (Reduced and Non-reduced): Using osteoinductive cells, i.e. BFP and Saos-2.
Immunocytochemistry: Using osteoinductive cells, i.e. BFP and Saos-2.
Immunogen
Recombinant protein corresponding to mouse BMP4
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
(Immunolocalization of BMP4 (red) in the lateral ventricle area of P7 mouse CNS. The blue stain is Hoechst nuclear counter stain. Photo courtesy of Dr. Jason Emsley, Harvard Medical School, Boston, MA.)
Testing Data
(Immunolocalization of BMP4 (red) in the lateral ventricle area of P7 mouse CNS. The blue stain is Hoechst nuclear counter stain. Photo courtesy of Dr. Jason Emsley, Harvard Medical School, Boston, MA.)
Related Product Information for anti-Bone Morphogenetic Protein 4 antibody
Bone Morphogenic Protein 4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signalling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord. It acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin or follistatin) causes the ectoderm to differentiate into the neural plate. If these cells also receive signals from FGF, they will differentiate into the spinal cord; in the absence of FGF the cells become brain tissue.
Product Categories/Family for anti-Bone Morphogenetic Protein 4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
bone morphogenic protein 4
Protein Family
Similar Products
Product Notes
The Bone Morphogenetic Protein 4 (Catalog #AAA600501) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Bone Morphogenetic Protein 4 (BMP4, DVR4) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Bone Morphogenetic Protein 4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC). Suitable for use in ELISA, Western Blot and Immunocytochemistry. Dilution: Western Blot (Reduced and Non-reduced): Using osteoinductive cells, i.e. BFP and Saos-2. Immunocytochemistry: Using osteoinductive cells, i.e. BFP and Saos-2. Researchers should empirically determine the suitability of the Bone Morphogenetic Protein 4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bone Morphogenetic Protein 4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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