BMP-4 elisa kit

Bovine BMP-4 PicoKine ELISA Kit

Cat. Num. AAA1751028

• Gene Names: BMP4; ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
• Reactivity: Bovine; There is no detectable cross-reactivity with other relevant proteins.
• Applications: ELISA
• Synonyms: BMP-4; Bovine BMP-4 PicoKine ELISA Kit; Bone morphogenetic protein 4; Bone morphogenetic protein 2B; BMP-2B; BMP4; BMP2B; DVR4; BMP-4 elisa kit

• Reactivity: Bovine; There is no detectable cross-reactivity with other relevant proteins.
• Specificity: Natural and recombinant Bovine Bovine BMP-4
• Sequence Length: 408

• Applicable Applications for BMP-4 elisa kit: ELISA (EIA)
• Assay Type: Quantitative Sandwich
• Detection Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <4pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration weretested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration weretested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP-4 elisa kit

Principle of the Assay: Bovine Bovine BMP-4 ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Bovine BMP-4 has been precoated onto 96-well plates. Standards(Expression system for standard: NSO; Immunogen sequence: S293-R408) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Bovine BMP-4 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Bovine Bovine BMP-4 amount of sample captured in plate.

Background: Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23.1, The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In Bovine embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog(released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal(by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. The standard product used in this kit is recombinant BMP-4 with the molecular mass of 26KDa.

Product Categories/Family for BMP-4 elisa kit

Cardiovascular; Angiogenesis; Growth Factors; TGF; Signal Transduction; Growth Factors/Hormones; Stem Cells; Signaling Pathways; TGF Beta; Secreted; Cancer; Developmental Biology; Organogenesis; Gut Development; Gut Specification; Nervous System Developme

NCBI and Uniprot Product Information

• NCBI GI #: 157276593
• NCBI GeneID: 652
• NCBI Accession #: NP_001193.2
• NCBI GenBank Nucleotide #: NM_001202.5
• UniProt Accession #: P12644; Q9UM80
• Molecular Weight: 46555 MW
• NCBI Official Full Name: bone morphogenetic protein 4 isoform a preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 4
• NCBI Official Symbol: BMP4
• NCBI Official Synonym Symbols: ZYME; BMP2B; OFC11; BMP2B1; MCOPS6
• NCBI Protein Information: bone morphogenetic protein 4
• UniProt Protein Name: Bone morphogenetic protein 4
• UniProt Gene Name: BMP4
• UniProt Synonym Gene Names: BMP2B; DVR4; BMP-4; BMP-2B

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates heart development and adipogenesis. Mutations in this gene are associated with orofacial cleft and microphthalmia in human patients. The encoded protein may also be involved in the pathology of multiple cardiovascular diseases and human cancers. [provided by RefSeq, Jul 2016]

Uniprot Description

Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction ().