Mouse BMP4 ELISA Kit | BMP4 elisa kit

Mouse BMP4 ELISA Kit

Cat. Num. AAA8248420

• Gene Names: Bmp4; Bmp-4; Bmp2b; Bmp2b1; Bmp2b-1
• Reactivity: Mouse
• Synonyms: BMP4; Mouse BMP4 ELISA Kit; BMP2B; DVR4; Bone morphogenetic protein 4; BMP-4; Bone morphogenetic protein 2B; BMP-2B; BMP4 elisa kit

• Reactivity: Mouse
• Specificity: The Mouse BMP4 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse BMP4 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
• Sequence Length: 408

• Samples: Bone Tissue, Cell Culture Supernates, Serum
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP4 elisa kit

Principle of the Assay: The MyBioSource' Mouse BMP4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse BMP4 in Bone Tissue, Cell Culture Supernates, Serum. This assay employs an antibody specific for Mouse BMP4 coated on a 96-well plate. Standards and samples are pipetted into the wells and BMP4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse BMP4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of BMP4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.!!Background/Introduction: Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23. The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-beta superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate. The standard product used in this kit is recombinant BMP-4 with the molecular mass of 26KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 939320322
• NCBI GeneID: 12159
• NCBI Accession #: NP_001303289.1
• NCBI GenBank Nucleotide #: NM_001316360.1
• UniProt Accession #: P21275
• Molecular Weight: 46,497 Da
• NCBI Official Full Name: bone morphogenetic protein 4 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 4
• NCBI Official Symbol: Bmp4
• NCBI Official Synonym Symbols: Bmp-4; Bmp2b; Bmp2b1; Bmp2b-1
• NCBI Protein Information: bone morphogenetic protein 4
• UniProt Protein Name: Bone morphogenetic protein 4
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: Bmp4
• UniProt Synonym Gene Names: Bmp-4; Dvr-4; BMP-4; BMP-2B
• UniProt Entry Name: BMP4_MOUSE

NCBI Description

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate each subunit of the disulfide-linked homodimer. This protein regulates heart development and adipogenesis. Homozygous knockout mice die in utero, while a conditional knockout mouse exhibits defects in heart development. Transgenic mice overexpressing this gene in a neuron-specific manner exhibit a phenotype resembling the rare hereditary connective tissue disease, fibrodysplasia ossificans progressiva. [provided by RefSeq, Jul 2016]

Uniprot Description

BMP4: Induces cartilage and bone formation. Also act in mesoderm induction, tooth development, limb formation and fracture repair. Acts in concert with PTHLH/PTHRP to stimulate ductal outgrowth during embryonic mammary development and to inhibit hair follicle induction. Homodimer; disulfide-linked. Interacts with GREM2. Part of a complex consisting of TWSG1 and CHRD. Interacts with the serine proteases, HTRA1 and HTRA3; the interaction with either inhibits BMP4-mediated signaling. The HTRA protease activity is required for this inhibition. Interacts with SOSTDC1. Expressed in the lung and lower levels seen in the kidney. Present also in normal and neoplastic prostate tissues, and prostate cancer cell lines. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: cytoplasm; extracellular region; extracellular space; vesicle

Molecular Function: chemoattractant activity; cytokine activity; growth factor activity; heparin binding; protein binding; protein homodimerization activity; transforming growth factor beta receptor binding

Biological Process: activation of MAPKK activity; alveolus development; anatomical structure formation; angiogenesis; anterior/posterior axis specification; blood vessel development; blood vessel endothelial cell proliferation during sprouting angiogenesis; BMP signaling pathway; branching morphogenesis of a tube; camera-type eye development; camera-type eye morphogenesis; cardiac muscle cell differentiation; cell fate commitment; chondrocyte differentiation; cloacal septation; common-partner SMAD protein phosphorylation; dorsoventral neural tube patterning; embryonic cranial skeleton morphogenesis; embryonic digit morphogenesis; embryonic hindlimb morphogenesis; embryonic limb morphogenesis; embryonic morphogenesis; embryonic skeletal development; embryonic skeletal morphogenesis; endochondral ossification; endoderm development; erythrocyte differentiation; eye development; forebrain development; germ cell development; heart development; hemopoietic progenitor cell differentiation; induction of an organ; inner ear receptor cell differentiation; intermediate mesodermal cell differentiation; kidney development; lung development; lymphoid progenitor cell differentiation; macrophage differentiation; mesoderm formation; mesodermal cell differentiation; mesodermal cell fate determination; metanephros development; monocyte differentiation; negative regulation of apoptosis; negative regulation of cell cycle; negative regulation of cell proliferation; negative regulation of chondrocyte differentiation; negative regulation of epithelial cell proliferation; negative regulation of immature T cell proliferation in the thymus; negative regulation of MAP kinase activity; negative regulation of mitosis; negative regulation of myoblast differentiation; negative regulation of oligodendrocyte differentiation; negative regulation of phosphorylation; negative regulation of striated muscle development; negative regulation of T cell differentiation in the thymus; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; neural tube closure; neuron fate commitment; odontogenesis; odontogenesis of dentine-containing teeth; osteoblast differentiation; ovarian follicle development; pituitary gland development; positive regulation of apoptosis; positive regulation of BMP signaling pathway; positive regulation of bone mineralization; positive regulation of cardiac muscle fiber development; positive regulation of cell differentiation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of collagen biosynthetic process; positive regulation of endothelial cell differentiation; positive regulation of endothelial cell proliferation; positive regulation of epidermal cell differentiation; positive regulation of epithelial cell proliferation; positive regulation of neuron differentiation; positive regulation of ossification; positive regulation of osteoblast differentiation; positive regulation of protein amino acid phosphorylation; positive regulation of protein binding; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of vascular endothelial growth factor receptor signaling pathway; post-embryonic development; regulation of cell differentiation; regulation of cell fate commitment; regulation of endothelial cell proliferation; regulation of gene expression; regulation of odontogenesis of dentine-containing teeth; regulation of protein import into nucleus; regulation of smooth muscle cell differentiation; regulation of smooth muscle cell proliferation; renal system process; skeletal development; smooth muscle cell differentiation; smooth muscle development; specification of organ position; steroid hormone mediated signaling; telencephalon development; telencephalon regionalization; tissue development; ureteric bud branching; ureteric bud development; vasculature development