Mouse Reelin Monoclonal Antibody | anti-RELN antibody

Reelin (RELN, RL, Reeler)

Cat. Num. AAA601699

• Gene Names: RELN; RL; LIS2; PRO1598
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Reelin; Monoclonal Antibody; Reelin (RELN; RL; Reeler); Anti -Reelin (RELN; anti-RELN antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 9C52
• Specificity: Recognizes mouse Reelin. Species Crossreactivity: rat, human. Reacts poorly with non-rodent Reelin.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-RELN antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry.; Dilution: Western Blot: 1:500 detects a band at~ 388kD; Immunohistochemistry: paraffin sections
• Immunogen: Recombinant fusion protein, corresponding to aa164-496 of mouse Reelin (derived from E15-E17 embryonic brain). Cellular Localization: Secreted.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(MBS601699 staining of embryonic cerebral cortex at 15 days in utero, with the pial surface above and the ventricle below. The positive zone is the Cajal-Retzius cell layer in the marginal zone, positive for reelin usung MBS601699.)

Testing Data

(MBS601699. Immunolocalization of Reelin (red) in the cortex of e14.5 mouse CNS. The blue stain is Hoechst nuclear counter stain. Photo courtesy of Dr. Jason Emsley, Harvard Medical School, Boston, MA.)

Related Product Information for anti-RELN antibody

The reeler mutant in mouse displays an abnormal pattern of cell migration throughout the cerebral and cerebellar cortices. The preplate forms normally, and the neurons differentiate at the correct times in the ventricular zone. However, instead of forming the normal "inside-out" arrangement of neurons in the cortical plate, the older neurons are found furthest from the ventricular zone, while the younger neurons do not migrate far at all. The reeler cerebral cortex is inverted from that of the wild type mouse.

Product Categories/Family for anti-RELN antibody

Antibodies; Abs to Extracellular Matrix Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 21464487
• NCBI GeneID: 5649
• UniProt Accession #: P78509; Q86UJ0; Q86UJ8; Q8NDV0; Q9UDQ2; A4D0P9; A4D0Q0; Q75MM8
• Molecular Weight: 388,388 Da
• NCBI Official Full Name: reelin
• NCBI Official Synonym Full Names: reelin
• NCBI Official Symbol: RELN
• NCBI Official Synonym Symbols: RL; LIS2; PRO1598
• NCBI Protein Information: reelin; OTTHUMP00000211830; OTTHUMP00000211831
• UniProt Protein Name: Reelin
• Protein Family: Reelin
• UniProt Gene Name: RELN
• UniProt Entry Name: RELN_HUMAN

NCBI Description

This gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorder, major depression and in migration defects associated with temporal lobe epilepsy. Mutations of this gene are associated with autosomal recessive lissencephaly with cerebellar hypoplasia. Two transcript variants encoding distinct isoforms have been identified for this gene. Other transcript variants have been described but their full length nature has not been determined. [provided by RefSeq]

Uniprot Description

reelin: Extracellular matrix serine protease that plays a role in layering of neurons in the cerebral cortex and cerebellum. Regulates microtubule function in neurons and neuronal migration. Affects migration of sympathetic preganglionic neurons in the spinal cord, where it seems to act as a barrier to neuronal migration. Enzymatic activity is important for the modulation of cell adhesion. Binding to the extracellular domains of lipoprotein receptors VLDLR and LRP8/APOER2 induces tyrosine phosphorylation of DAB1 and modulation of TAU phosphorylation. Defects in RELN are the cause of lissencephaly type 2 (LIS2); also known as lissencephaly with cerebellar hypoplasia or Norman-Roberts syndrome. LIS2 is a classic type lissencephaly associated with abnormalities of the cerebellum, hippocampus and brainstem. Individuals with LIS2 are severely ataxic, mentally retarded and suffer from epilepsy. Belongs to the reelin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; EC 3.4.21.-; Motility/polarity/chemotaxis; Protease; Cell development/differentiation; Secreted; Cell adhesion

Chromosomal Location of Human Ortholog: 7q22

Cellular Component: extracellular space; proteinaceous extracellular matrix; cytoplasm; dendrite

Molecular Function: serine-type peptidase activity; metal ion binding; protein serine/threonine/tyrosine kinase activity

Biological Process: cellular morphogenesis during differentiation; axon guidance; peptidyl-tyrosine phosphorylation; central nervous system development; spinal cord patterning; neuron migration; response to pain; proteolysis; positive regulation of synaptic transmission, glutamatergic; glial cell differentiation; dendrite development; cell adhesion; associative learning; layer formation in the cerebral cortex; positive regulation of TOR signaling pathway; regulation of behavior; regulation of synaptic transmission; cerebral cortex tangential migration; hippocampus development; activation of CREB transcription factor; ventral spinal cord development; positive regulation of small GTPase mediated signal transduction; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of peptidyl-tyrosine phosphorylation; long-term memory; positive regulation of protein kinase activity; brain development

Disease: Epilepsy, Familial Temporal Lobe, 7; Lissencephaly 2; Epilepsy, Familial Temporal Lobe, 1