Rabbit anti-Human Bax Monoclonal Antibody | anti-Bax antibody

Bax RMab

Cat. Num. AAA370130

• Reactivity: Human
• Synonyms: Bax; Monoclonal Antibody; Bax RMab; anti-Bax antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: E63
• Form/Format: Bax is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

• Control: Breast, Tonsil, Cervix,Hodgkin’s Lymphoma
• Localization: Cytoplasmic, Membranous
• Immunogen: Synthetic peptide within Human Bax aa 1-100 (N-terminal).
• Reactivity Note: Paraffin, Frozen
• Staining Procedure: 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides ; 2. Air dry for 2 hours at 58° C. ; 3. Deparaffinize, dehydrate and rehydrate tissues. ; 4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate . ; ; 5. Any of three heating methods may be used: ; ; a. TintoRetriever r Pressure Cooker or Equivalent; Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.; b. TintoRetriever PT Module or Water Bath Method ; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA ; Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.; c. Conventional Steamer Method; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA ; Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes. ; ; 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes. ; 7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions. ; 8. Wash slides with IHC wash buffer or DI water. ; 9. Continue IHC staining protocol
• Precautions: 1. For professional users only. Ensure results are interpreted by a medical professional.; 2. This product contains sodium azide (NaN3), a toxic chemical which may react with plumbing to form highly explosive build-ups of metal azides. Upon disposal, ?ush with large volumes of water to prevent sodium azide build-up.; 3. Ensure proper handling procedures are used with reagent. Always wear proper laboratory equipment such as laboratory coat and gloves when handling reagents.; 4. Unused solution should be disposed of according to local and federal regulations.; 5. Do not ingest reagent. If reagent ingested, contact a poison control center immediately
• Preparation and Storage: Store at 2-8°C. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropiately when not in use, and avoid prolonged exposure to room temperature conditions.

Dilution Information

Immunohistochemistry (IHC)

Related Product Information for anti-Bax antibody

Bax is a protein of the bcl-2 gene family. It promotes apoptosis by competing with bcl-2 proper. The Bax gene contains a small promoter element that complements a binding domain on the multi-faceted p53 tumor suppressor. Wild-type p53 has been demonstrated to upregulate the transcription of a chimeric reporter plasmid, utilizing the consensus promoter sequence of Bax approx. 50-fold over mutant p53. Mutations in this consensus sequence eliminate transcription of the reporter gene. Thus, it is likely that p53 promotes Bax's apoptotic faculties in vivo as a primary transcription factor. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Bax targets mitochondrial mem-branes, inducing mitochondrial damage and cell death in a caspase-independent manner. Bad plays a critical role in the Bax-mediated apoptosis pathway by dimerizing with BclxL, causing the displacement of Bax. The displacement of Bax allows apoptosis to proceed.

NCBI and Uniprot Product Information

• NCBI GI #: 975870
• NCBI GeneID: 24887
• UniProt Accession #: Q63690; Q62995; Q64383; Q9JKL3
• NCBI Official Full Name: bax
• NCBI Official Synonym Full Names: Bcl2-associated X protein
• NCBI Official Symbol: Bax
• NCBI Protein Information: apoptosis regulator BAX
• UniProt Protein Name: Apoptosis regulator BAX
• Protein Family: Bax inhibitor
• UniProt Gene Name: Bax
• UniProt Entry Name: BAX_RAT

NCBI Description

Bcl2-related gene; involved in the regulation of apoptotic cell death [RGD]

Uniprot Description

Function: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.

Subunit structure: Homodimer. Forms higher oligomers under stress conditions. Interacts with BCL2L11. Interaction with BCL2L11 promotes BAX oligomerization and association with mitochondrial membranes, with subsequent release of cytochrome c. Forms heterodimers with BCL2, E1B 19K protein, BCL2L1 isoform Bcl-X(L), BCL2L2, MCL1 and A1. Interacts with SH3GLB1 and HN. Interacts with SFN and YWHAZ; the interaction occurs in the cytoplasm. Under stress conditions, JNK-mediated phosphorylation of SFN and YWHAZ, releases BAX to mitochondria. Interacts with RNF144B, which regulates the ubiquitin-dependent stability of BAX. Interacts with CLU under stress conditions that cause a conformation change leading to BAX oligomerization and association with mitochondria. Does not interact with CLU in unstressed cells

Subcellular location: Isoform Alpha: Mitochondrion outer membrane; Single-pass membrane protein

By similarity. Cytoplasm

By similarity. Note: Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane

By similarity.Isoform Beta: Cytoplasm

By similarity. Isoform Gamma: Cytoplasm

Tissue specificity: Expressed in a wide variety of tissues, with highest levels in the testis and ovary.

Domain: Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family

Sequence similarities: Belongs to the Bcl-2 family.