Mouse anti-Human PD-1 Monoclonal Antibody | anti-PD-1 antibody

PD-1/CD279

Cat. Num. AAA370123

• Reactivity: Human
• Synonyms: PD-1; Monoclonal Antibody; PD-1/CD279; anti-PD-1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: NAT-105
• Form/Format: PD-1 is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

• Application Notes: Concentrated: 1:100-1:500 (volume) 1.0ml
• Control: Tonsil, Lymph Node, Thymus, Spleen
• Localization: Cytoplasmic, Membranous
• Reactivity Note: Paraffin, Frozen
• Preservative: The sodium azide (NaN3) used as a preservative, is toxic if ingested.
• Staining Procedure: 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides ; 2. Air dry for 2 hours at 58° C. ; 3. Deparaffinize, dehydrate and rehydrate tissues. ; 4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate . ; ; 5. Any of three heating methods may be used: ; ; a. TintoRetriever r Pressure Cooker or Equivalent; Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.; b. TintoRetriever PT Module or Water Bath Method ; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA ; Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.; c. Conventional Steamer Method; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA ; Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes. ; ; 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes. ; 7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions. ; 8. Wash slides with IHC wash buffer or DI water. ; 9. Continue IHC staining protocol
• Precautions: 1. For professional users only. Ensure results are interpreted by a medical professional.; 2. This product contains sodium azide (NaN3), a toxic chemical which may react with plumbing to form highly explosive build-ups of metal azides. Upon disposal, ?ush with large volumes of water to prevent sodium azide build-up.; 3. Ensure proper handling procedures are used with reagent. Always wear proper laboratory equipment such as laboratory coat and gloves when handling reagents.; 4. Unused solution should be disposed of according to local and federal regulations.; 5. Do not ingest reagent. If reagent ingested, contact a poison control center immediately.
• Preparation and Storage: Store at 2-8°C. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.

Testing Data (TD)

Testing Data (TD)

Related Product Information for anti-PD-1 antibody

Programmed Death 1 (PD-1 or CD279) is a Type I membrane protein comprised of 268 amino acids. PD-1 is a member of the extended CD28/CTLA-4 family of T-cell regulators. PD-1 is expressed on the surface of activated T-cells, B-cells, and macrophages. In comparison to CTLA-4, PD-1 more broadly negatively regulates immune responses. New data suggests that expression of PD-L1 on tumor cells inhibits anti-tumor activity through engagement f PD-1 on effector T-cells. Expression of PD-L1 on tumors is correlated with reduced survival in esophageal, pancreatic and other types of cancers, highlighting the relevance of exploring the PD-1 pathway as a target for immunotherapy. Studies have found that PD-1 is expressed on most T-cells and a small subset of B-cells in the light zone of germinal centers, but not elsewhere in the tonsil. On that basis, it was postulated that PD-1 may play a role in the process of clonal selection of centrocytes, which occurs in this subanatomic site in germinal centers. PD-1 is a new marker of Angioimmunoblastic Lymphoma and suggests a unique cell of origin for this neoplasm. Unlike CD10 and bcl-6, PD-1 is expressed by few B-cells, so it may be a more specific and useful marker in Angioimmunoblastic Lymphoma. It also seems to stain a greater percentage of CD3-positive neoplastic cells in Angioimmunoblastic Lymphoma than either CD10 or bcl-6.