Mouse anti-Human Alpha Synuclein Monoclonal Antibody | anti-SNCA antibody

Alpha Synuclein Mmab

Cat. Num. AAA370326

• Gene Names: SNCA; PD1; NACP; PARK1; PARK4
• Reactivity: Human
• Synonyms: Alpha Synuclein; Monoclonal Antibody; Alpha Synuclein Mmab; anti-SNCA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a/K
• Sequence Length: 13

• Immunogen: Recombinant protein of the human alpha synuclein.
• Localization: Cytoplasmic, Nuclear
• Control: Brain, Breast, Skin, Tonsil, Bone Marrow, Alzheimer’s Disease, Parkinson’s Disease.
• Precautions: 1. This product contains sodium azide (NaN3), a toxic chemical which may react with plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent sodium azide build-up. ; 2. Ensure proper handling procedures are used with reagent. Always wear proper laboratory equipment such as laboratory coat and gloves when handling reagents. ; 3. Unused solution should be disposed of according to local and federal regulations. ; 4. Do not ingest reagent. If reagent ingested, contact a poison control center immediately. ; 5. For complete recommendations for handling biological specimens please refer to the CDC document(8).
• Specimen Preparation: Paraffin sections: The antibody can be used on formalin-fixed paraffinembedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation to ensure best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using MBS ImmunoDNA Retriever with Citrate, ImmunoDNA Retriever with EDTA (MBS) or ImmunoDNA Digestor. See reverse side for complete protocol. Tissue should remain hydrated via use of MBS Immuno/DNA Washer solutions. Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.
• Preparation and Storage: Store at 2-8 degree C. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.

Presentation

(Alpha Synuclein is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodiumazide as a preservative.)

Staining Procedure

(1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides.2. Air dry for 2 hours at 58° C.3. Deparaffinize, dehydrate and rehydrate tissues.4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA.5. Any of three heating methods may be used:a. TintoRetriever Pressure Cooker or EquivalentPlace tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature.b. TintoRetriever PT Module or Water Bath MethodPlace tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes.c. Conventional Steamer MethodPlace tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions.8. Wash slides with IHC wash buffer or DI water. 9. Continue IHC staining protocol.Recommended IHC Protocol)

IHC

(Inset: IHC of Alpha Synuclein on a FFPE Astrocytoma Tissue)

Related Product Information for anti-SNCA antibody

Alpha-synuclein is a 140 amino acids protein encoded by the SNCA gene. It is predominantly expressed in the neocortex, hippocampus, substantia nigra, thalamus, and cerebellum with smaller amounts are found in the heart, muscles, and other tissues. In the brain, alpha-synuclein is found mainly in presynaptic terminals.

An alpha-synuclein fragment, known as the non-Abeta component (NAC) of Alzheimer’s disease amyloid, originally found in an amyloid-enriched fraction, was shown to be a fragment of its precursor protein, NACP. Alpha-synuclein aggregates to form insoluble fibrils in pathological conditions characterized by Lewy bodies, such as Parkinson’s disease, dementia with Lewy bodies and multiple system atrophy. These disorders are known as synucleinopathies. Occasionally, Lewy bodies contain tau protein; however, alpha-synuclein and tau constitute two distinctive subsets of filaments in the same inclusion bodies.

Alpha-synuclein pathology is also found in both sporadic and familial cases with Alzheimer’s disease. In rare cases of familial forms of Parkinson’s disease, there is a mutation in the gene coding for alpha-synuclein. Genomic duplication and triplication of the gene appear to be a rare cause of Parkinson’s disease in other lineages, although more common than point mutations. Hence certain mutations of alpha-synuclein may cause it to form amyloid-like fibrils that contribute to Parkinson’s disease.

NCBI and Uniprot Product Information

• NCBI GI #: 46242542
• NCBI GeneID: 6622
• Molecular Weight: 13,109 Da
• NCBI Official Full Name: alpha synuclein, partial
• NCBI Official Synonym Full Names: synuclein alpha
• NCBI Official Symbol: SNCA
• NCBI Official Synonym Symbols: PD1; NACP; PARK1; PARK4
• NCBI Protein Information: alpha-synuclein
• UniProt Protein Name: Alpha-synuclein
• Protein Family: Alpha-synuclein
• UniProt Gene Name: SNCA
• UniProt Synonym Gene Names: NACP; PARK1; NACP
• UniProt Entry Name: SYUA_HUMAN

NCBI Description

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. [provided by RefSeq, Feb 2016]

Uniprot Description

SNCA: a member of the synuclein family. Abundantly expressed in the brain. Inhibits phospholipase D2 selectively. May integrate presynaptic signaling and membrane trafficking. Implicated in the pathogenesis of Parkinson's disease. A major component of amyloid plaques in the brains of patients with Alzheimer's disease. Two alternatively spliced isoforms transcripts have been identified.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: actin cytoskeleton; axon; cell cortex; cytoplasm; cytosol; extracellular region; extracellular space; fibril; growth cone; inclusion body; lysosome; membrane; mitochondrial respiratory chain complex I; mitochondrion; nucleus; perinuclear region of cytoplasm; plasma membrane; platelet alpha granule membrane

Molecular Function: alpha-tubulin binding; calcium ion binding; caspase inhibitor activity; copper ion binding; dynein binding; fatty acid binding; ferrous iron binding; histone binding; identical protein binding; kinesin binding; magnesium ion binding; oxidoreductase activity; phospholipid binding; phosphoprotein binding; protein binding; tau protein binding; zinc ion binding

Biological Process: calcium ion homeostasis; caspase activation; cellular protein metabolic process; dopamine biosynthetic process; dopamine uptake; fibril organization and biogenesis; microglial cell activation; negative regulation of apoptosis; negative regulation of caspase activity; negative regulation of dopamine uptake; negative regulation of exocytosis; negative regulation of histone acetylation; negative regulation of microtubule polymerization; negative regulation of monooxygenase activity; negative regulation of norepinephrine uptake; negative regulation of serotonin uptake; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transporter activity; positive regulation of apoptosis; positive regulation of endocytosis; positive regulation of peptidyl-serine phosphorylation; positive regulation of receptor recycling; positive regulation of release of sequestered calcium ion into cytosol; protein destabilization; receptor internalization; regulation of dopamine secretion; response to iron(II) ion; response to lipopolysaccharide; response to magnesium ion; synaptic vesicle endocytosis

Disease: Dementia, Lewy Body; Parkinson Disease 1, Autosomal Dominant; Parkinson Disease 4, Autosomal Dominant