Mouse GFAP Monoclonal Antibody | anti-GFAP antibody

GFAP

Cat. Num. AAA370073

• Reactivity: Human, Canine, Feline, Rat, Mouse, Rabbit
• Synonyms: GFAP; Monoclonal Antibody; anti-GFAP antibody

• Host: Mouse
• Reactivity: Human, Canine, Feline, Rat, Mouse, Rabbit
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: G-A-5
• Form/Format: GFAP is a mouse monoclonal antibody derived from cell culture supernatant that is concentrated, dialyzed, filter sterilized and diluted in buffer pH 7.5, containing BSA and sodium azide as a preservative.

• Control: Brain
• Localization: Cytoplasmic
• Immunogen: Purified GFAP isolated from porcine spinal cord.
• Reactivity Note: Paraffin, Frozen
• Precautions: 1. This product contains sodium azide (NaN3), a toxic chemical which may react with plumbing to form highly explosive build-ups of metal azides. Upon disposal, flush with large volumes of water to prevent sodium azide build-up.; 2. Ensure proper handling procedures are used with reagent. Always wear proper laboratory equipment such as laboratory coat and gloves when handling reagents.; 3. Unused solution should be disposed of according to local and federal regulations.; 4. Do not ingest reagent. If reagent ingested, contact a poison control center immediately.; 5. For complete recommendations for handling biological specimens please refer to the CDC document, “Guidelines for Safe Work Practices in Human and Animal Laboratories” (5).
• Specimen Preparation: Paraffin sections: The antibody can be used on formalin-fixed paraffin-embedded (FFPE) tissue sections. Ensure tissue undergoes appropriate fixation to ensure best results. Pre-treatment of tissues with heat-induced epitope retrieval (HIER) is recommended using ImmunoDNA Retriever with Citrate , ImmunoDNA Retriever with EDTA or ImmunoDNA Digestor . See reverse side for complete protocol. Tissue should remain hydrated via use of Immuno/DNA Washer solutions. Frozen sections and cell preparations: The antibody can be used for labeling acetone-fixed frozen sections and acetone-fixed cell preparations.
• Staining Procedure: 1. Cut and mount 3-5 micron formalin-fixed paraffin-embedded tissues on positive charged slides such as Hydrophilic Plus Slides.; 2. Air dry for 2 hours at 58° C.; 3. Deparaffinize, dehydrate and rehydrate tissues. ; 4. Subject tissues to heat epitope retrieval using a suitable retrieval solution such as ImmunoDNA Retriever with Citrate or EDTA.; 5. Any of three heating methods may be used: ; a. TintoRetriever Pressure Cooker or Equivalent; Place tissues/slides in a staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA, and place in the pressure cooker. Add 1-2 inches of distilled water to the pressure cooker and turn heat to high. Incubate for 15 minutes. Open and immediately transfer slides to room temperature. ; ; b. TintoRetriever PT Module or Water Bath Method; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA at 95°-99° C. Incubate for 30-60 minutes. ; ; c. Conventional Steamer Method; Place tissues/slides in a pre-warmed staining dish or coplin jar containing the ImmunoDNA Retriever with Citrate or EDTA in a Steamer, cover and steam for 30-60 minutes.; ; 6. After heat treatment, transfer slides in ImmunoDNA Retriever with Citrate or EDTA to room temperature and let stand for 15-20 minutes.; 7. For manual staining, perform antibody incubation at ambient temperature. For automated staining methods, perform antibody incubation according to instrument manufacturer’s instructions.; 8. Wash slides with IHC wash buffer or DI water.; 9. Continue IHC staining protocol.
• Preparation and Storage: Store at 2-8 °C. Do not use after expiration date listed on package label. Temperature fluctuations should be avoided. Store appropriately when not in use, and avoid prolonged exposure to room temperature conditions.

Presentation

Immunohistochemistry (IHC)

Recommended Protocol

Related Product Information for anti-GFAP antibody

Glial fibrillary acidic protein or GFAP is a Type III protein of the intermediate filaments principally found in astrocytes in the central nervous system, but can also be found in neurons, hepatic stellate cells, kidney mesangial cells, pancreatic stellatecells, and Leydig cells. It has a role in the cytoskeleton of the astrocyte and possibly many other stellate-shaped cells. Antibodies to GFAP are very useful as markers of astrocytic cells. In addition, many types of brain tumors, presumably derived from astrocytic cells, heavily express GFAP. This marker is mainly used to distinguish neoplasms of astrocytic origin from other neoplasms in the central nervous system.

NCBI and Uniprot Product Information

• NCBI GI #: 251802
• NCBI Official Full Name: glial fibrillary acidic protein
• Protein Family: Glial fibrillary acidic protein

NCBI Description

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

Uniprot Description

GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: membrane; cytoplasm; intermediate filament; cytosol

Molecular Function: integrin binding; structural constituent of cytoskeleton; kinase binding

Biological Process: Bergmann glial cell differentiation; extracellular matrix organization and biogenesis; regulation of neurotransmitter uptake; response to wounding; neurite regeneration; intermediate filament organization; astrocyte development

Disease: Alexander Disease