General Trehalase Assay Kit | TREH assay kit

Trehalase Assay Kit

Cat. Num. AAA822353

• Gene Names: TREH; TRE; TREA
• Reactivity: General
• Applications: Functional Assay
• Synonyms: Trehalase; Trehalase Assay Kit; TREH assay kit

• Reactivity: General
• Sequence Length: 583

• Applicable Applications for TREH assay kit: Functional Assay
• Preparation and Storage: Shipped and store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TREH assay kit

Background/Introduction: Transglutaminases (EC 2.3.2.13) are calcium dependent enzymes that catalyze the post-translational modification of proteins by formation of isopeptide bonds. This occurs either through protein cross-linking via formation of gamma-glutamyl-epsilon-lysine bonds or through incorporation of primary amines at selected peptide-bound glutamine residues. The transglutaminase enzyme family comprises the intracellular forms (TG1, TG3 and TG5) expressed mostly in the epithelial tissue; TG2 which is both intracellular and extracellular and expressed in various tissue types; TG4 which is expressed in the prostate gland; factor XIII which is expressed in blood; TG6 and TG7, whose tissue distribution is unknown and band 4.2 (lacking enzymatic activity) which is present on erythrocyte membranes. Transglutaminases also exhibit GTPase, phosphodiesterase and protein kinase activity. Transglutaminases are associated with certain neurological and autoimmune disorders and also cancer. MBS, Inc. Transglutaminase Activity Assay kit utilizes the deamidation reaction of the transglutaminase enzyme with a donor and acceptor substrate resulting in the formation of a hydroxamate product. The hydroxamate product reacts with the Stop Solution forming a purple complex that can be measured colorimetrically at 525 nm. The limit of quantification of this assay is ~10 uU or 80 ng of recombinant hTG2 enzyme.

NCBI and Uniprot Product Information

• NCBI GI #: 116284412
• NCBI GeneID: 11181
• NCBI Accession #: NP_009111.2
• NCBI GenBank Nucleotide #: NM_007180.2
• Molecular Weight: 62,941 Da
• NCBI Official Full Name: trehalase isoform 1
• NCBI Official Synonym Full Names: trehalase (brush-border membrane glycoprotein)
• NCBI Official Symbol: TREH
• NCBI Official Synonym Symbols: TRE; TREA
• NCBI Protein Information: trehalase; alpha,alpha-trehalose glucohydrolase
• UniProt Protein Name: Trehalase
• Protein Family: Trehalase
• UniProt Gene Name: TREH
• UniProt Synonym Gene Names: TREA
• UniProt Entry Name: TREA_HUMAN

NCBI Description

This gene encodes an enzyme that hydrolyses trehalose, a disaccharide formed from two glucose molecules found mainly in fungi, plants, and insects. A partial duplication of this gene is located adjacent to this locus on chromosome 11. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Uniprot Description

TREH: Intestinal trehalase is probably involved in the hydrolysis of ingested trehalose. Deficiency of TREH results in isolated trehalose intolerance that causes gastrointestinal symptoms after ingestion of edible mushrooms. Belongs to the glycosyl hydrolase 37 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.2.1.28; Membrane protein, GPI anchor; Carbohydrate Metabolism - starch and sucrose; Hydrolase

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: anchored to plasma membrane; plasma membrane

Molecular Function: alpha,alpha-trehalase activity

Biological Process: polysaccharide digestion; organ morphogenesis; trehalose catabolic process; trehalose metabolic process; carbohydrate metabolic process; pathogenesis

Disease: Trehalase Deficiency