Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Rabbit PDLI3 Polyclonal Antibody | anti-PDLI3 antibody
PDLI3 Antibody
Gene Names
PDLIM3; ALP
Reactivity
Human, Mouse, RatPredicted: Dog
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
PDLI3; Polyclonal Antibody; PDLI3 Antibody; Actinin associated LIM protein; Actinin-associated LIM protein; Alpha actinin 2 associated LIM protein; Alpha-actinin-2-associated LIM protein; DKFZp686L0362; Enigma homolog; PDLI3_HUMAN; PDLIM 3; PDLIM3; PDZ and LIM domain 3; PDZ and LIM domain protein 3; ALP; anti-PDLI3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Dog
Predicted: Dog
Clonality
Polyclonal
Isotype
IgG
Specificity
ALP Antibody detects endogenous levels of ALP.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
316
Applicable Applications for anti-PDLI3 antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human ALP.
Tissue Specificity
Isoform 1 is highly expressed in differentiated skeletal muscle. Isoform 2 is heart-specific.
Fragment
Fab Fragment
Conjugate
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(1:200 Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(1:600 Green) were used as the secondary antibod)
Western Blot (WB)
(Western blot analysis of extracts from Human heart tissue, using ALP Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Human heart tissue, using ALP Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Mouse spleen, using ALP Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from Mouse spleen, using ALP Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-PDLI3 antibody
Description: May play a role in the organization of actin filament arrays within muscle cells.
Subunit Structure: Interacts with ACTN2 (By similarity). Forms a heterodimer with PDLIM4 (via LIM domain) (By similarity).
Subunit Structure: Interacts with ACTN2 (By similarity). Forms a heterodimer with PDLIM4 (via LIM domain) (By similarity).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 35; 39 kDa
Predicted: 40 kDa
Predicted: 40 kDa
NCBI Official Full Name
PDZ and LIM domain protein 3 isoform b
NCBI Official Synonym Full Names
PDZ and LIM domain 3
NCBI Official Symbol
PDLIM3
NCBI Official Synonym Symbols
ALP
NCBI Protein Information
PDZ and LIM domain protein 3
UniProt Protein Name
PDZ and LIM domain protein 3
UniProt Gene Name
PDLIM3
UniProt Synonym Gene Names
ALP
UniProt Entry Name
PDLI3_HUMAN
NCBI Description
The protein encoded by this gene contains a PDZ domain and a LIM domain, indicating that it may be involved in cytoskeletal assembly. In support of this, the encoded protein has been shown to bind the spectrin-like repeats of alpha-actinin-2 and to colocalize with alpha-actinin-2 at the Z lines of skeletal muscle. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Aberrant alternative splicing of this gene may play a role in myotonic dystrophy. [provided by RefSeq, Apr 2012]
Research Articles on PDLI3
Similar Products
Product Notes
The PDLI3 pdlim3 (Catalog #AAA9611277) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDLI3 Antibody reacts with Human, Mouse, Rat Predicted: Dog and may cross-react with other species as described in the data sheet. AAA Biotech's PDLI3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PDLI3 pdlim3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDLI3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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