Rabbit Actin Polyclonal Antibody | anti-ACTB antibody

Actin (Tyr-53), Phospho-Specific

Cat. Num. AAA474080

• Gene Names: ACTB; BRWS1; PS1TP5BP1
• Reactivity: Human, Rat, Mouse, Chicken
• Applications: Western Blot, ELISA, Immunocytochemistry
• Purity: Affinity Purified
• Synonyms: Actin; Polyclonal Antibody; Actin (Tyr-53); Phospho-Specific; anti-ACTB antibody

• Host: Rabbit
• Reactivity: Human, Rat, Mouse, Chicken
• Clonality: Polyclonal
• Specificity: This antibody detects a 42 kDa* protein corresponding to the molecular mass of Actin on SDS-PAGE immunoblots of pervanadate treated human C2C12 and SYF cSrc transformed cells, but not in control cells. In addition, this antibody either detects actin cleavage products at 36 and 20 kDa, or cross-reacts with unidentified proteins at these molecular weights in C2C12 cells.; ; All molecular weights (MW) are confirmed by comparison to MW standards and to western blot mobilites of known proteins with similar MW. "Native" western blots utilizes non-reducing sample buffer (no mercaptoethanol or SDS), normal SDS-PAGE gel electrophoresis, and no methanol in transfer buffers.
• Purity/Purification: Affinity Purified
• Form/Format: Supplied in 100ul phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
• Sequence Length: 375

• Applicable Applications for anti-ACTB antibody: Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC)
• Application Notes: WB: 1:1000; ICC: 1:50; EIA: 1:2000; ; IMPORTANT: For western blots, incubate membrane with diluted antibody in 5% w/v milk , 1X TBS, 0.1% Tween®20 at 4°C with gentle shaking, overnight.
• Immunogen: Phospho-Actin (Tyr-53) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 53 of human β actin. This sequence is identical to similar regions in all four α actins, as well as in γ actin, and is well conserved in actins from most eukaryotic species.
• Preparation and Storage: Store at -20°C. Stable for 1 year.

Western Blot (WB)

(Western blot analysis of mouse C2C12 cells untreated (lanes 1 & 3), or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). The blot was probed with anti-Actin (N-terminal) antibody (lanes 1 & 2) or anti-Actin (Tyr-53) antibody (lanes 3 & 4).)

Immunocytochemistry (ICC)

( Immunocytochemical labeling using anti-Actin (N-terminal) and anti-Actin (Tyr-53) polyclonal antibodies in C2C12 cells control (left) or treated with pervanadate (1 mM) for 30 min (middle). The cells were fixed in paraformaldehyde and permeabilized in acetone. Both antibodies were used in the presence of blocking peptide: Actin (N-terminal) peptide (MBS474001) or phospho-Actin (Tyr-53) peptide, respectively (right).)

Related Product Information for anti-ACTB antibody

Actin is a major cytoskeletal protein involved in diverse cellular functions including cell motility, adhesion, and morphology. Six different actin isoforms have been identified in vertebrates. There are four alpha isoforms: skeletal, cardiac, and two smooth muscle (enteric and aortic) actins, along with two cytoplasmic actins (beta and gamma). Actin exists in two principal forms, globular, monomeric (G) actin, and filamentous polymeric (F) actin. The assembly and disassembly of actin filaments, and also their organization into functional networks, is regulated by a variety of actin-binding proteins (ABPs). Phosphorylation may also be important for regulating actin assembly and interaction with ABPs. In Dictyostelium, phosphorylation of Tyr-53 occurs in response to cell stress and this phosphorylation may alter actin polymerization. In B cells, SHP-1 tyrosine dephosphorylation of actin leads to actin filament depolymerization following BCR stimulation.

References

Vonach, C. et al. (2011) British J Cancer. 105:263. Jungbluth, A. et al. (1995) FEBS Let. 375:87.Baba, T. et al. (2003) J. Immunol. 170: 3762.Winder, S.J. et al. (2005) J. Cell Sci. 118:651.Liu, X. et al. (2006) Proc Nat Acad Sci U S A. 103(37):13694.

NCBI and Uniprot Product Information

• NCBI GI #: 4501885
• NCBI GeneID: 60
• NCBI Accession #: NP_001092.1
• NCBI GenBank Nucleotide #: NM_001101.5
• UniProt Accession #: P60709
• NCBI Official Full Name: actin, cytoplasmic 1
• NCBI Official Synonym Full Names: actin beta
• NCBI Official Symbol: ACTB
• NCBI Official Synonym Symbols: BRWS1; PS1TP5BP1
• NCBI Protein Information: actin, cytoplasmic 1
• UniProt Protein Name: Actin, cytoplasmic 1
• Protein Family: Actin
• UniProt Gene Name: ACTB
• UniProt Entry Name: ACTB_HUMAN

NCBI Description

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]