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Chromatin Immunoprecipitation (ChIP)
(Histone H3K36me3 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from a human glioma cell line. ChIP DNA was sequenced on the Illumina NextSeq and 16.8 million sequence tags were mapped to identify H3K36me3 binding on chromosome 7. The antibody was used at 4 ug per ChIP.)
Rabbit Histone H3K36me3 Polyclonal Antibody | anti-H3K36me3 antibody
Histone H3K36me3 antibody (pAb)
Reactivity
Human, Mouse, Wide Range Predicted
Applications
Chromatin Immunoprecipitation, Immunoprecipitation, Chromatin Immunoprecipitation, Immunoprecipitation, Dot Blot, Western Blot
Purity
Protein A Chromatography
Synonyms
Histone H3K36me3; Polyclonal Antibody; Histone H3K36me3 antibody (pAb); anti-H3K36me3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Wide Range Predicted
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Protein A Chromatography
Form/Format
Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Concentration
1ug/ul (varies by lot)
Applicable Applications for anti-H3K36me3 antibody
Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Cleavage Under Targets and Tagmentation (CUT&Tag), Dot Blot (DB), Western Blot (WB)
Application Notes
Applications Validated:
ChIP: 10ug per ChIP
ChIP-Seq: 4ug each WB: 0.5-2ug/ml dilution CUT&Tag: 1ug per 50ul reaction For Histone H3K36me3, we also offer AbFlex Histone H3K36me3 Recombinant Antibody (rAb). For details
ChIP: 10ug per ChIP
ChIP-Seq: 4ug each WB: 0.5-2ug/ml dilution CUT&Tag: 1ug per 50ul reaction For Histone H3K36me3, we also offer AbFlex Histone H3K36me3 Recombinant Antibody (rAb). For details
Conjugation
Methylated
Immunogen
This Histone H3 trimethyl Lys36 antibody was raised against a peptide containing trimethyl Lys36 of human histone H3.
Abs Residue
Lys
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul
Chromatin Immunoprecipitation (ChIP)
(Histone H3K36me3 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from a human glioma cell line. ChIP DNA was sequenced on the Illumina NextSeq and 16.8 million sequence tags were mapped to identify H3K36me3 binding on chromosome 7. The antibody was used at 4 ug per ChIP.)
Chromatin Immunoprecipitation (ChIP)
(Histone H3K36me3 antibody (pAb) tested by ChIP-Seq. ChIP was performed using the ChIP-IT High Sensitivity Kit with 30 ug of chromatin from a human glioma cell line. ChIP DNA was sequenced on the Illumina NextSeq and 16.8 million sequence tags were mapped to identify H3K36me3 binding on chromosome 7. The antibody was used at 4 ug per ChIP.)
Chromatin Immunoprecipitation (ChIP)
(Chromatin IP: ChIP performed using HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) and 10 ug of Histone H3 trimethyl Lys36 antibody (pAb) or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for either the PABPC1 (+ 0.5) or the PABPC1 ( +18) gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Chromatin Immunoprecipitation (ChIP)
(Chromatin IP: ChIP performed using HeLa Chromatin (1.5 x 106 cell equivalents per ChIP) and 10 ug of Histone H3 trimethyl Lys36 antibody (pAb) or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for either the PABPC1 (+ 0.5) or the PABPC1 ( +18) gene. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)
Western Blot (WB)
(Western blot of Histone H3K36me3 pAb. Nuclear extract of HeLa cells (20 ug) probed with Histone H3K36me3 antibody at a dilution of 0.1 ug/ml.)
Western Blot (WB)
(Western blot of Histone H3K36me3 pAb. Nuclear extract of HeLa cells (20 ug) probed with Histone H3K36me3 antibody at a dilution of 0.1 ug/ml.)
Dot Blot (DB)
(Dot blot specificity analysis of Histone H3K26me3 pAb: Dot blot analysis was used to confirm the specificity of Histone H3K36me3 antibody for trimethyl-lysine 36 of histone H3. Peptides corresponding to regions around major sites of histone H3 methylation were spotted onto PVDF and probed with the antibody at a dilution of 1 ug/ml. The amount of peptide (in picomoles) spotted is indicated next to each row. Top panel - Lane 1: unmodified Lys4. Lane 2: K4me1. Lane 3: K4me1. Lane 4: K4me2. Lane 5: K4me3. Lane 6: unmodified Lys9. Lane 7: K9me1. Lane 8: K9me2. Lane 9: K9me3. Lower panel - Lane 1: unmodified Lys27. Lane 2: K27me1. Lane 3: K27me2. Lane 4: K27me3. Lane 5: unmodified Lys36. Lane 6: K36me1. Lane 7: K36me2. Lane 8: K36me3.)
Dot Blot (DB)
(Dot blot specificity analysis of Histone H3K26me3 pAb: Dot blot analysis was used to confirm the specificity of Histone H3K36me3 antibody for trimethyl-lysine 36 of histone H3. Peptides corresponding to regions around major sites of histone H3 methylation were spotted onto PVDF and probed with the antibody at a dilution of 1 ug/ml. The amount of peptide (in picomoles) spotted is indicated next to each row. Top panel - Lane 1: unmodified Lys4. Lane 2: K4me1. Lane 3: K4me1. Lane 4: K4me2. Lane 5: K4me3. Lane 6: unmodified Lys9. Lane 7: K9me1. Lane 8: K9me2. Lane 9: K9me3. Lower panel - Lane 1: unmodified Lys27. Lane 2: K27me1. Lane 3: K27me2. Lane 4: K27me3. Lane 5: unmodified Lys36. Lane 6: K36me1. Lane 7: K36me2. Lane 8: K36me3.)
Related Product Information for anti-H3K36me3 antibody
Short Description: Our Histone H3K36me3 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Chromatin Immunoprecipitation, ChIP-Seq, CUT&Tag, Dot blot and Western blot; it has been shown to react with Human and Mouse samples, but it is predicted that it will react with a wide range of sample types.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes.
Background: Histone H3 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation; these modifications play a major role in regulating gene expression. The methylation of histones can occur on two different residues: arginine or lysine. Histone methylation can be associated with transcriptional activation or repression, depending on the methylated residue. Histone H3 is methylated at lysine 36 by the Set2 (yeast) and NSD1 (mammals) methyltransferases. Dimethylation of lysine 36 of histone H3 is involved with transcriptional elongation by RNA pol II holoenzyme and is a marker of transcribed genes.
Product Categories/Family for anti-H3K36me3 antibody
NCBI and Uniprot Product Information
Molecular Weight
17kDa
Similar Products
Product Notes
The H3K36me3 (Catalog #AAA388759) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Histone H3K36me3 antibody (pAb) reacts with Human, Mouse, Wide Range Predicted and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H3K36me3 can be used in a range of immunoassay formats including, but not limited to, Chromatin Immunoprecipitation (ChIP), Chromatin Immunoprecipitation-Seq (ChIP-Seq), Cleavage Under Targets and Tagmentation (CUT&Tag), Dot Blot (DB), Western Blot (WB). Applications Validated: ChIP: 10ug per ChIP ChIP-Seq: 4ug each WB: 0.5-2ug/ml dilution CUT&Tag: 1ug per 50ul reaction For Histone H3K36me3, we also offer AbFlex Histone H3K36me3 Recombinant Antibody (rAb). For details. Researchers should empirically determine the suitability of the H3K36me3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H3K36me3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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