Rat MMP-8 PicoKine ELISA Kit | Mmp8 elisa kit

Rat MMP-8 PicoKine ELISA Kit

Cat. Num. AAA177648

• Reactivity: Rat
• Synonyms: MMP-8 PicoKine; Rat MMP-8 PicoKine ELISA Kit; Neutrophil collagenase; BB138268; CLG 1; CLG1; Collagenase 1; Collagenase 1 neutrophil; HNC; Matrix metallopeptidase 8; Matrix metalloproteinase 8 (neutrophil collagenase); Matrix metalloproteinase-8; MMP 8; MMP-8; Mmp8; MMP8_HUMAN; PMNL CL; PMNL collagenase; PMNL-CL; matrix metallopeptidase 8 (neutrophil collagenase); Mmp8 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat MMP-8
• Sequence Length: 466

• Assay Range: 156pg/ml-10,000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of rat MMP-8 in cell culture supernates, serum and plasma(heparin).
• Immunogen: NSO,L21-P466
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 10,000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml rat MMP-8 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin) to each empty well. It is recommended that each rat MMP-8 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|486|1780|6826|561|2175|7203; Standard deviation|19.93|112.14|354.95|27.49|158.78|468.2; CV(%)|4.1|6.3|5.2|4.9|7.3|6.5
• Sample Data: Concentration(pg/ml)|0|156|312|625|1250|2500|5000|10000; O.D.|0.006|0.192|0.366|0.635|0.985|1.400|1.892|2.193
• Anticoagulant: Heparin
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Rat MMP-8 PicoKine ELISA Kit standard curve )

Related Product Information for Mmp8 elisa kit

Principle of the assay: Rat MMP-8 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for MMP-8 has been precoated onto 96-well plates. Standards(NSO, L21-P466) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat MMP-8 amount of sample captured in plate.

Background: Matrix metalloproteinase 8(MMP8) also called neutrophil collagenase. Neutrophil collagenase, a member of the family of matrix metalloproteinases, is distinct from the collagenase of skin fibroblasts and synovial cells in substrate specificity and immunologic crossreactivity. MMP8, an enzyme that degrades fibrillar collagens imparting strength to the fetal membranes, is expressed by leukocytes and chorionic cytotrophoblast cells. The human neutrophil collagenase(HNC) cDNA clone has been sequenced and shown to encode a 467-residue protein. Neutrophil collagenase has been found to possess 57% identity with the deduced protein sequence for fibroblast collagenase with 72% chemical similarity. Certain regions of the molecule, including the putative zinc-binding region, are highly conserved. When compared with the published sequence for fibroblast collagenase, neutrophil collagenase contains four additional sites for glycosylation. The standard product used in this kit is natural, isolating from human MMP-8. The detected MMP-8 includes zymogen and active enzyme.

NCBI and Uniprot Product Information

• NCBI GI #: 11560008
• NCBI GeneID: 63849
• NCBI Accession #: NP_071557.1
• NCBI GenBank Nucleotide #: NM_022221.1
• UniProt Accession #: O88766
• Molecular Weight: 53,277 Da
• NCBI Official Full Name: neutrophil collagenase
• NCBI Official Synonym Full Names: matrix metallopeptidase 8
• NCBI Official Symbol: Mmp8
• NCBI Protein Information: neutrophil collagenase
• UniProt Protein Name: Neutrophil collagenase
• Protein Family: Neutrophil collagenase
• UniProt Gene Name: Mmp8
• UniProt Synonym Gene Names: MMP-8
• UniProt Entry Name: MMP8_RAT

NCBI Description

mouse homolog is a neutral metalloproteinase of the fibrillar collagenase family [RGD, Feb 2006]

Uniprot Description

Can degrade fibrillar type I, II, and III collagens.