MMP-8 Recombinant Protein | MMP8 recombinant protein

MMP-8 Protein, Mouse, Recombinant

Cat. Num. AAA8122783

• Gene Names: Mmp8; BB138268
• Purity: >85% as determined by SDS-PAGE
• Synonyms: MMP-8; MMP-8 Protein; Mouse; Recombinant; Mouse MMP-8/CLG1 Protein; BB138268 Protein; matrix metallopeptidase 8; MMP8 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 50mM NaCl, 10mM CaCl2, 0.05% Brij 35, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Phe21-Ser465

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Met
• Tag: Native
• Protein Construction: A DNA sequence encoding the pro form of mouse MMP8 (NP_032637.3)(Phe 21-Ser 465) was expressed and purified, with an initial Met at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for MMP8 recombinant protein

Background: Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases that degrade components of the extracellular matrix (ECM) and play essential roles in various physiological processes such as morphogenesis, differentiation, angiogenesis, and tissue remodeling, as well as pathological processes including inflammation, arthritis, cardiovascular diseases, pulmonary diseases, and tumor invasion. Neutrophil collagenase, also known as Matrix metalloproteinase-8, MMP-8, and CLG1, is a member of the peptidase M1A family. MMP-8 may affect the metastatic behavior of breast cancer cells through protection against lymph node metastasis, underlining the importance of anti-target identification in drug development. MMP-8 in the tumor may have a protective effect against lymph node metastasis. MMP-8 may affect the metastatic behavior of breast cancer cells through protection against lymph node metastasis, underlining the importance of anti-target identification in drug development. MMP-8 participates in wound repair by contributing to the resolution of inflammation and open the possibility to develop new strategies for treating wound healing defects.

References

Hasty K.A., et al.,(1990), Human neutrophil collagenase. A distinct gene product with homology to other matrix metalloproteinases. J. Biol. Chem. 265:11421-11424.Blaeser J., et al., (1991), Mercurial activation of human polymorphonuclear leucocyte procollagenase.Eur. J. Biochem. 202:1223-1230.Blaeser J., et al.,(1991), Mercurial activation of human polymorphonuclear leucocyte procollagenase. Eur. J. Biochem. 202:1223-1230.

NCBI and Uniprot Product Information

• NCBI GI #: 160333381
• NCBI GeneID: 17394
• NCBI Accession #: NP_032637.3
• NCBI GenBank Nucleotide #: NM_008611.4
• UniProt Accession #: O70138; O88733; Q6GTR5
• Molecular Weight: 53,126 Da
• NCBI Official Full Name: neutrophil collagenase preproprotein
• NCBI Official Synonym Full Names: matrix metallopeptidase 8
• NCBI Official Symbol: Mmp8
• NCBI Official Synonym Symbols: BB138268
• NCBI Protein Information: neutrophil collagenase
• UniProt Protein Name: Neutrophil collagenase
• Protein Family: Neutrophil collagenase
• UniProt Gene Name: Mmp8
• UniProt Synonym Gene Names: MMP-8
• UniProt Entry Name: MMP8_MOUSE

NCBI Description

This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme that degrades types I, II and III collagens. Mice lacking the encoded protein exhibit abnormalities in the inflammatory responses to various agents. This gene is located in a cluster of other matrix metalloproteinase genes on chromosome 9. [provided by RefSeq, Feb 2016]

Uniprot Description

MMP8: Can degrade fibrillar type I, II, and III collagens. Belongs to the peptidase M10A family.

Protein type: Secreted, signal peptide; Secreted; EC 3.4.24.34; Protease

Cellular Component: extracellular matrix; extracellular region; extracellular space; proteinaceous extracellular matrix

Molecular Function: calcium ion binding; hydrolase activity; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; serine-type endopeptidase activity; zinc ion binding

Biological Process: collagen catabolic process; proteolysis