Typical Testing Data/Standard Curve (for reference only)
Rat Matrix metalloproteinase-14 ELISA Kit | Mmp14 elisa kit
Rat Matrix metalloproteinase-14 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP-14. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MMP-14 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MMP-14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MMP-14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
enzyme which is an activator of progelatinase A [RGD, Feb 2006]
Uniprot Description
MMP14: Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Up-regulated by NANOS1. Expressed in stromal cells of colon, breast, and head and neck. Expressed in lung tumors. Belongs to the peptidase M10A family.
Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Protease; EC 3.4.24.80
Cellular Component: cytoplasm; cytoplasmic vesicle; extracellular matrix; focal adhesion; integral to membrane; melanosome; plasma membrane
Molecular Function: calcium ion binding; integrin binding; metalloendopeptidase activity; protease activator activity; zinc ion binding
Biological Process: angiogenesis; astrocyte cell migration; branching morphogenesis of a tube; cell migration; collagen catabolic process; embryonic cranial skeleton morphogenesis; endochondral ossification; endothelial cell proliferation; lung development; male gonad development; negative regulation of focal adhesion formation; negative regulation of Notch signaling pathway; ossification; ovarian follicle development; positive regulation of B cell differentiation; positive regulation of cell growth; positive regulation of cell migration; proteolysis; response to estrogen stimulus; response to hormone stimulus; response to hypoxia; response to mechanical stimulus; response to organic cyclic substance; response to oxidative stress; tissue remodeling; zymogen activation
Research Articles on Mmp14
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Product Notes
The Rat Mmp14 mmp14 (Catalog #AAA2881455) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2881455 ELISA Kit recognizes Rat Mmp14. It is sometimes possible for the material contained within the vial of "Matrix metalloproteinase-14, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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