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Typical Testing Data/Standard Curve (for reference only)

Rat MMP14 (Matrix metalloproteinase-14) ELISA Kit | MMP14 elisa kit

Rat MMP14 (Matrix metalloproteinase-14) ELISA Kit

Gene Names
Mmp14; Mt1-mmp
Reactivity
Rat
Synonyms
MMP14 (Matrix metalloproteinase-14); Rat MMP14 (Matrix metalloproteinase-14) ELISA Kit; MMP-14; Matrix metalloproteinase-14; Membrane-type matrix metalloproteinase 1; MT-MMP 1; MTMMP1; Membrane-type-1 matrix metalloproteinase; T-MMP; MT1-MMP; MT1MMP; Mmp14; Mtmmp; MMP14 elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of MMP14. No significant cross-reactivity or interference between MMP14 and analogues was observed.
Samples
Serum, plasma, tissue homogenates and other biological fluids
Assay Type
Quantitative Sandwich
Detection Range
31.25-2000pg/ml
Sensitivity
18.75pg/ml
Intra-assay Precision
CV<8%
Inter-assay Precision
CV<10%
Preparation and Storage
Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MMP14 elisa kit
Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
66,080 Da
NCBI Official Full Name
matrix metalloproteinase-14
NCBI Official Synonym Full Names
matrix metallopeptidase 14
NCBI Official Symbol
Mmp14
NCBI Official Synonym Symbols
Mt1-mmp
NCBI Protein Information
matrix metalloproteinase-14
UniProt Protein Name
Matrix metalloproteinase-14
Protein Family
UniProt Gene Name
Mmp14
UniProt Synonym Gene Names
Mtmmp; MMP-14; MT-MMP 1; MTMMP1; MT-MMP; MT1-MMP; MT1MMP

NCBI Description

enzyme which is an activator of progelatinase A [RGD, Feb 2006]

Uniprot Description

Endopeptidase that degrades various components of the extracellular matrix such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development. May be involved in actin cytoskeleton reorganization by cleaving PTK7. Acts as a positive regulator of cell growth and migration via activation of MMP15 in association with pro-MMP2. Involved in the formation of the fibrovascular tissues in association with pro-MMP2. Cleaves ADGRB1 to release vasculostatin-40 which inhibits angiogenesis.

Research Articles on MMP14

Similar Products

Product Notes

The Rat MMP14 mmp14 (Catalog #AAA7612438) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7612438 ELISA Kit recognizes Rat MMP14. It is sometimes possible for the material contained within the vial of "MMP14 (Matrix metalloproteinase-14), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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