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Western Blot (WB)
(Western Blot: Sample: Recombinant TCF20, Mouse.)
Rabbit anti-Mouse Transcription factor 20 (TCF20) Polyclonal Antibody | anti-TCF20 antibody
Polyclonal Antibody to Transcription factor 20 (TCF20)
Gene Names
Tcf20; SPBP; mKIAA0292; 2810438H08Rik
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Transcription factor 20 (TCF20); Polyclonal Antibody; Polyclonal Antibody to Transcription factor 20 (TCF20); anti-TCF20 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against TCF20. It has been selected for its ability to recognize TCF20 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF- ELPLDSNEFW VHEGCILWAN GIYLVCGRLY GLQEALEIAR EMKCSHCQEA GATLGCYNKG CSFRYHYPCA IDADCLLHEE NFSVRCPKHK VRLWR
MGHHHHHHSGSEF- ELPLDSNEFW VHEGCILWAN GIYLVCGRLY GLQEALEIAR EMKCSHCQEA GATLGCYNKG CSFRYHYPCA IDADCLLHEE NFSVRCPKHK VRLWR
Sequence Length
1968
Applicable Applications for anti-TCF20 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant TCF20 (Glu1871~Arg1965) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2039681
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Recombinant TCF20, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant TCF20, Mouse.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Testis Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Testis Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Heart Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Heart Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
213,566 Da
NCBI Official Full Name
transcription factor 20 isoform a
NCBI Official Synonym Full Names
transcription factor 20
NCBI Official Symbol
Tcf20
NCBI Official Synonym Symbols
SPBP; mKIAA0292; 2810438H08Rik
NCBI Protein Information
transcription factor 20
UniProt Protein Name
Transcription factor 20
Protein Family
UniProt Gene Name
Tcf20
UniProt Synonym Gene Names
Spbp; TCF-20; SPRE-binding protein
Uniprot Description
TCF20: a transcriptional activator. Binds to the regulatory region of MMP3 and thereby controls stromelysin expression. It stimulates the activity of various transcriptional activators such as JUN, SP1, PAX6 and ETS1, suggesting a function as a coactivator. Two alternatively spliced isoforms have been described. Isoform 1 is exclusively expressed in brain, heart and testis, and predominates in liver and kidney. Isoform 2 predominates in lung.
Protein type: DNA-binding; Nuclear receptor co-regulator; Transcription factor
Chromosomal Location of Human Ortholog: 15|15 E1
Cellular Component: nuclear body; nucleoplasm; nucleus
Molecular Function: DNA binding; DNA binding transcription factor activity; metal ion binding; protein binding; RNA binding; transcription regulatory region DNA binding
Biological Process: positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-templated; transcription, DNA-dependent
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Product Notes
The TCF20 tcf20 (Catalog #AAA2004194) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Transcription factor 20 (TCF20) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Transcription factor 20 (TCF20) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the TCF20 tcf20 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF- ELPLDSNEFW VHEGCILWAN GIYLVCGRLY GLQEALEIAR EMKCSHCQEA GATLGCYNKG CSFRYHYPCA IDADCLLHEE NFSVRCPKHK VRLWR. It is sometimes possible for the material contained within the vial of "Transcription factor 20 (TCF20), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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