Mouse Matrix metalloproteinase-23 ELISA Kit | Mmp23 elisa kit

Mouse Matrix metalloproteinase-23 ELISA Kit

Cat. Num. AAA288398

• Gene Names: Mmp23; CA-MMP
• Reactivity: Mouse
• Synonyms: Matrix metalloproteinase-23; Mouse Matrix metalloproteinase-23 ELISA Kit; Mmp23 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse MMP-23. No significant cross-reactivity or interference was observed.
• Sequence Length: 390

• Assay Type: Sandwich
• Detection Range: 0.78-50 ng/mL
• Sensitivity: 0.41 ng/mL
• Intra-Assay CV: <=6.3%
• Inter-Assay CV: <=10.9%
• Recovery: 105%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Mmp23 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP-23. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for MMP-23 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain MMP-23, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of MMP-23 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6754710
• NCBI GeneID: 26561
• NCBI Accession #: NP_036115.1
• NCBI GenBank Nucleotide #: NM_011985.3
• UniProt Accession #: O88676; Q3KNC0
• Molecular Weight: 42,946 Da
• NCBI Official Full Name: matrix metalloproteinase-23 isoform 1
• NCBI Official Synonym Full Names: matrix metallopeptidase 23
• NCBI Official Symbol: Mmp23
• NCBI Official Synonym Symbols: CA-MMP
• NCBI Protein Information: matrix metalloproteinase-23
• UniProt Protein Name: Matrix metalloproteinase-23
• Protein Family: Matrix metalloproteinase
• UniProt Gene Name: Mmp23
• UniProt Synonym Gene Names: Cammp; MMP-23; CA-MMP
• UniProt Entry Name: MMP23_MOUSE

NCBI Description

This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded protein contains an N-terminal cysteine array and a novel immunoglobulin-fold domain at the C-terminus. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing. [provided by RefSeq, Feb 2016]

Uniprot Description

MMP23B: Protease. May regulate the surface expression of some potassium channels by retaining them in the endoplasmic reticulum. Belongs to the peptidase M10A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Protease; EC 3.4.24.-

Cellular Component: endoplasmic reticulum; extracellular matrix; integral to membrane; membrane

Molecular Function: hydrolase activity; metal ion binding; metalloendopeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding

Biological Process: proteolysis