Typical Testing Data/Standard Curve (for reference only)
Mouse Matrix Metalloproteinase 14 (MMP14) ELISA Kit | MMP14 elisa kit
Mouse Matrix Metalloproteinase 14 (MMP14) ELISA Kit
Gene Names
Mmp14; sabe; MMP-X1; MT1-MMP; AI325305; MT-MMP-1
Reactivity
Mouse
Synonyms
Matrix Metalloproteinase 14 (MMP14); Mouse Matrix Metalloproteinase 14 (MMP14) ELISA Kit; MMP-X1; MT1-MMP; MTMMP1; Membrane Inserted; Membrane-type matrix metalloproteinase 1; Membrane-type-1 matrix metalloproteinase; MMP14 elisa kit
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of MMP14. No significant cross-reactivity or interference between MMP14 and analogues was observed.
Sequence Length
582
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Assay Type
Sandwich
Detection Range
93.75-6000pg/mL
Sensitivity
32.81pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MMP14 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MMP14 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Preparation and Storage
For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MMP14 elisa kit
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of MMP14 in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP14. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MMP14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MMP14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MMP14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MMP14. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MMP14. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MMP14, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of MMP14 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
65,919 Da
NCBI Official Full Name
matrix metalloproteinase-14 preproprotein
NCBI Official Synonym Full Names
matrix metallopeptidase 14 (membrane-inserted)
NCBI Official Symbol
Mmp14
NCBI Official Synonym Symbols
sabe; MMP-X1; MT1-MMP; AI325305; MT-MMP-1
NCBI Protein Information
matrix metalloproteinase-14
UniProt Protein Name
Matrix metalloproteinase-14
Protein Family
UniProt Gene Name
Mmp14
UniProt Synonym Gene Names
Mtmmp; MMP-14; MT-MMP 1; MTMMP1; MT1-MMP; MT1MMP
NCBI Description
This gene encodes a member of the matrix metalloproteinase family of extracellular matrix-degrading enzymes that are involved in tissue remodeling, wound repair, progression of atherosclerosis and tumor invasion. The encoded preproprotein undergoes proteolytic processing to generate a mature, zinc-dependent endopeptidase enzyme. Mice lacking the encoded protein exhibit craniofacial dysmorphism, arthritis, osteopenia, dwarfism, and fibrosis of soft tissues. [provided by RefSeq, Feb 2016]
Uniprot Description
Endopeptidase that degrades various components of the extracellular matrix, such as collagen. Activates progelatinase A. Essential for pericellular collagenolysis and modeling of skeletal and extraskeletal connective tissues during development. May be involved in actin cytoskeleton reorganization by cleaving PTK7 (). Acts as a positive regulator of cell growth and migration via activation of MMP15. Involved in the formation of the fibrovascular tissues ().
Research Articles on MMP14
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Product Notes
The Mouse MMP14 mmp14 (Catalog #AAA453931) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA453931 ELISA Kit recognizes Mouse MMP14. It is sometimes possible for the material contained within the vial of "Matrix Metalloproteinase 14 (MMP14), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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