Mouse Microtubule-associated protein tau ELISA Kit | Mapt elisa kit

Mouse Microtubule-associated protein tau ELISA Kit

Cat. Num. AAA2886657

• Gene Names: Mapt; Tau; Mtapt; AI413597; AW045860
• Reactivity: Mouse
• Synonyms: Microtubule-associated protein tau; Mouse Microtubule-associated protein tau ELISA Kit; Neurofibrillary tangle protein; Paired helical filament-tau; PHF-tau; Mapt; Mtapt; Tau; Mapt elisa kit

• Reactivity: Mouse
• Sequence Length: 430

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Mapt elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve

NCBI and Uniprot Product Information

• NCBI GI #: 84370347
• NCBI GeneID: 17762
• NCBI Accession #: NP_001033698.1
• NCBI GenBank Nucleotide #: NM_001038609.2
• UniProt Accession #: P10637; P10638; Q60684; Q60685; Q60686; Q62286; Q91WK4; A2A5Y9
• Molecular Weight: 36,740 Da
• NCBI Official Full Name: microtubule-associated protein tau isoform a
• NCBI Official Synonym Full Names: microtubule-associated protein tau
• NCBI Official Symbol: Mapt
• NCBI Official Synonym Symbols: Tau; Mtapt; AI413597; AW045860
• NCBI Protein Information: microtubule-associated protein tau
• UniProt Protein Name: Microtubule-associated protein tau
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: Mapt
• UniProt Synonym Gene Names: Mtapt; Tau; PHF-tau
• UniProt Entry Name: TAU_MOUSE

Uniprot Description

Tau: a microtubule-associated protein that regulates microtubule assembly and stability. Apparently involved in the establishment and maintenance of neuronal polarity. Mutations can result in several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. Nine differentially spliced isoforms have been described. The short isoforms allow plasticity of the cytoskeleton, whereas the longer isoforms may preferentially play a role in its stabilization.

Protein type: Cytoskeletal

Cellular Component: axon; axoneme; cell projection; cytoplasm; cytoskeleton; dendrite; growth cone; membrane; microtubule; microtubule associated complex; microtubule cytoskeleton; neuron projection; nucleus; plasma membrane; postsynaptic density; tubulin complex

Molecular Function: apolipoprotein binding; enzyme binding; Hsp90 protein binding; microtubule binding; protein binding; protein complex binding; protein domain specific binding; protein kinase binding; protein phosphatase 2A binding; SH3 domain binding; tubulin binding

Biological Process: adult walking behavior; apoptosis; axon cargo transport; axon extension; axonogenesis; induction of apoptosis by oxidative stress; microtubule cytoskeleton organization and biogenesis; mitochondrion transport along microtubule; negative regulation of intracellular transport; neuron migration; positive regulation of axon extension; positive regulation of microtubule polymerization; regulation of autophagy; response to nutrient; response to organic substance