Mouse Microtubule Associated Protein Tau (MAPt) ELISA Kit | MAPt elisa kit

Mouse Microtubule Associated Protein Tau (MAPt) ELISA Kit

Cat. Num. AAA2021160

• Gene Names: MAPT; tau
• Reactivity: Mouse
• Synonyms: Microtubule Associated Protein Tau (MAPt); Mouse Microtubule Associated Protein Tau (MAPt) ELISA Kit; DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; Neurofibrillary tangle protein; Tau Protein; Paired helical filament-tau; G Protein Beta1/Gamma2 Subunit-Interacting Factor 1; MAPt elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Microtubule Associated Protein Tau (MAPt).; No significant cross-reactivity or interference between Microtubule Associated Protein Tau (MAPt) and analogues was observed.
• Sequence Length: 448

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.059ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Microtubule Associated Protein Tau (MAPt) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Microtubule Associated Protein Tau (MAPt) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAPt elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microtubule Associated Protein Tau (MAPt). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microtubule Associated Protein Tau (MAPt). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microtubule Associated Protein Tau (MAPt), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Microtubule Associated Protein Tau (MAPt) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MAPt elisa kit

Metabolic pathway; Neuroscience; Developmental science

NCBI and Uniprot Product Information

• NCBI GI #: 27806547
• NCBI GeneID: 281296
• NCBI Accession #: NP_776531.1
• NCBI GenBank Nucleotide #: NM_174106.2
• UniProt Accession #: P29172; P29173; Q28185; Q28186; Q28187; Q28188; Q28189; Q28190; Q32KT2
• Molecular Weight: 43,806 Da
• NCBI Official Full Name: microtubule-associated protein tau
• NCBI Official Symbol: MAPT
• NCBI Official Synonym Symbols: tau
• NCBI Protein Information: microtubule-associated protein tau
• UniProt Protein Name: Microtubule-associated protein tau
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: MAPT
• UniProt Synonym Gene Names: TAU; PHF-tau
• UniProt Entry Name: TAU_BOVIN

Uniprot Description

Tau: a microtubule-associated protein that regulates microtubule assembly and stability. Apparently involved in the establishment and maintenance of neuronal polarity. Mutations can result in several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. Nine differentially spliced isoforms have been described. The short isoforms allow plasticity of the cytoskeleton, whereas the longer isoforms may preferentially play a role in its stabilization.

Protein type: Cytoskeletal

Cellular Component: microtubule; growth cone; axon; tubulin complex; plasma membrane; axoneme; cytosol

Molecular Function: protein binding; microtubule binding; apolipoprotein binding; SH3 domain binding; protein kinase binding

Biological Process: negative regulation of intracellular transport; axon extension; positive regulation of axon extension; positive regulation of microtubule polymerization; neuron migration; axon cargo transport; regulation of autophagy; microtubule cytoskeleton organization and biogenesis; adult walking behavior; mitochondrion transport along microtubule