Rabbit anti-Human TAF6 Polyclonal Antibody | anti-TAF6 antibody

TAF6, NT (TAF6, TAF2E, TAFII70, Transcription initiation factor TFIID subunit 6, RNA polymerase II TBP-associated factor subunit E, Transcription initiation factor TFIID 70kD subunit, Transcription initiation factor TFIID 80kD subunit) (PE)

Cat. Num. AAA6353412

• Gene Names: TAF6; TAF2E; TAFII70; TAFII80; TAFII85; MGC:8964; TAFII-70; TAFII-80; TAF(II)70; TAF(II)80
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TAF6; Polyclonal Antibody; NT (TAF6; TAF2E; TAFII70; Transcription initiation factor TFIID subunit 6; RNA polymerase II TBP-associated factor subunit E; Transcription initiation factor TFIID 70kD subunit; Transcription initiation factor TFIID 80kD subunit) (PE); Transcription initiation factor TFIID 80kD subunit; anti-TAF6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TAF6 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TAF6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-53 amino acids from the N-terminal region of human TAF6.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TAF6 Antibody (N-term) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the TAF6 antibody detected the TAF6 protein (arrow).)

Related Product Information for anti-TAF6 antibody

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Alternative splicing results in multiple transcript variants.

Product Categories/Family for anti-TAF6 antibody

Antibodies; Transcription Factors

References

Theisen, J.W., et al. Mol. Cell. Biol. (2010). In press: Wilhelm, E., et al. BMC Mol. Biol. 11, 10 (2010): Wilhelm, E., et al. PLoS ONE 3 (7), E2721 (2008): Frontini, M., et al. Mol. Cell. Biol. 25(11):4638-4649(2005). Beausoleil, S.A., et al. Proc. Natl. Acad. Sci. U.S.A. 101(33):12130-12135(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 298676521
• NCBI GeneID: 6878
• NCBI Accession #: NP_005632.1; NP_647476.1
• NCBI GenBank Nucleotide #: NM_005641
• UniProt Accession #: P49848; Q6AI29; A4D2B2; A4D2B3; B4DT11; D6W5U2
• Molecular Weight: 76,995 Da
• NCBI Official Full Name: Homo sapiens TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa (TAF6), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: TAF6 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 80kDa
• NCBI Official Symbol: TAF6
• NCBI Official Synonym Symbols: TAF2E; TAFII70; TAFII80; TAFII85; MGC:8964; TAFII-70; TAFII-80; TAF(II)70; TAF(II)80
• NCBI Protein Information: transcription initiation factor TFIID subunit 6; RNA polymerase II TBP-associated factor subunit E; TATA box binding protein (TBP)-associated factor, RNA polymerase II, E, 70/85kD; transcription initiation factor TFIID 70 kDa subunit
• UniProt Protein Name: Transcription initiation factor TFIID subunit 6
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF6
• UniProt Synonym Gene Names: TAF2E; TAFII70
• UniProt Entry Name: TAF6_HUMAN

NCBI Description

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the smaller subunits of TFIID that binds weakly to TBP but strongly to TAF1, the largest subunit of TFIID. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010]

Uniprot Description

TAF6: TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TIIFD is multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. Belongs to the TAF6 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 7q22.1

Cellular Component: nucleoplasm; transcription factor TFIID complex; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; protein heterodimerization activity; transcription factor activity

Biological Process: transcription from RNA polymerase II promoter; negative regulation of cell proliferation; transcription initiation from RNA polymerase II promoter; regulation of transcription, DNA-dependent; regulation of transcription factor activity; viral reproduction; RNA elongation from RNA polymerase II promoter; transcription initiation; gene expression; negative regulation of cell cycle