Rat Microtubule Associated Protein Tau (MAPt) RTU ELISA Kit | MAPt rtu elisa kit

Rat Microtubule Associated Protein Tau (MAPt) ELISA Kit

Cat. Num. AAA4500910

• Gene Names: Mapt; Tau; pTau; Mtapt; RNPTAU
• Reactivity: Rat
• Synonyms: Microtubule Associated Protein Tau (MAPt); Rat Microtubule Associated Protein Tau (MAPt) ELISA Kit; DDPAC; FTDP-17; MAPTL; MSTD; MTBT1; MTBT2; PPND; Neurofibrillary tangle protein; Tau Protein; Paired helical filament-tau; G Protein Beta1/Gamma2 Subunit-Interacting Factor 1; MAPt rtu elisa kit

• Reactivity: Rat
• Sequence Length: 752

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates, Cell Culture Supernates or Other Biological Fluids.
• Detection Range: 78.1-5000pg/mL
• Sensitivity: 25pg/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAPt rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAPt. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MAPt. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MAPt, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MAPt in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13432197
• NCBI GeneID: 29477
• UniProt Accession #: P19332; Q63567; Q63677; Q9QW06
• Molecular Weight: 81,185 Da
• NCBI Official Full Name: Microtubule-associated protein tau
• NCBI Official Synonym Full Names: microtubule-associated protein tau
• NCBI Official Symbol: Mapt
• NCBI Official Synonym Symbols: Tau; pTau; Mtapt; RNPTAU
• NCBI Protein Information: microtubule-associated protein tau
• UniProt Protein Name: Microtubule-associated protein tau
• Protein Family: Microtubule-associated protein
• UniProt Gene Name: Mapt
• UniProt Synonym Gene Names: Mtapt; Tau; PHF-tau

NCBI Description

a microtubule-associated protein; expression is found specifically in neurons [RGD, Feb 2006]

Uniprot Description

Promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of neuronal polarity. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization.