Rat phosphorylated microtubule Associated protein tau ELISA Kit | pMAPT/pTAU elisa kit

Rat phosphorylated microtubule Associated protein tau ELISA Kit

Cat. Num. AAA725984

• Gene Names: Mapt; pTau; Mtapt; RNPTAU
• Reactivity: Rat
• Synonyms: phosphorylated microtubule Associated protein tau; Rat phosphorylated microtubule Associated protein tau ELISA Kit; pMAPT/pTAU elisa kit

• Reactivity: Rat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/ml
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for pMAPT/pTAU elisa kit

Intended Uses: This pTAU ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat pTAU. This ELISA kit for research use only!

Principle of the Assay: pTAU ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-pTAU antibody and an pTAU-HRP conjugate. The assay sample and buffer are incubated together with pTAU-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the pTAU concentration since pTAU from samples and pTAU-HRP conjugate compete for the anti-pTAU antibody binding site. Since the number of sites is limited, as more sites are occupied by pTAU from the sample, fewer sites are left to bind pTAU-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The pTAU concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 672069501
• NCBI GeneID: 29477
• NCBI Accession #: XP_008766504.1
• NCBI GenBank Nucleotide #: XM_008768282.1
• Molecular Weight: 81,185 Da
• NCBI Official Full Name: microtubule-associated protein tau isoform X14
• NCBI Official Synonym Full Names: microtubule-associated protein tau
• NCBI Official Symbol: Mapt
• NCBI Official Synonym Symbols: pTau; Mtapt; RNPTAU
• NCBI Protein Information: microtubule-associated protein tau; PHF-tau; paired helical filament-tau; neurofibrillary tangle protein; Tau microtubule-associated protein
• UniProt Protein Name: Microtubule-associated protein tau
• UniProt Gene Name: Mapt
• UniProt Synonym Gene Names: Mtapt; Tau; PHF-tau
• UniProt Entry Name: TAU_RAT

NCBI Description

a microtubule-associated protein; expression is found specifically in neurons [RGD, Feb 2006]

Uniprot Description

Tau: a microtubule-associated protein that regulates microtubule assembly and stability. Apparently involved in the establishment and maintenance of neuronal polarity. Mutations can result in several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. The C-terminus binds axonal microtubules while the N-terminus binds neural plasma membrane components, suggesting that tau functions as a linker. Axonal polarity is predetermined by tau localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. Nine differentially spliced isoforms have been described. The short isoforms allow plasticity of the cytoskeleton, whereas the longer isoforms may preferentially play a role in its stabilization.

Protein type: Cytoskeletal

Cellular Component: microtubule; neuron projection; cell; postsynaptic density; cytosol; microtubule cytoskeleton; growth cone; axon; tubulin complex; cytoplasm; plasma membrane; axoneme; intracellular; nucleus

Molecular Function: protein domain specific binding; protein binding; enzyme binding; protein phosphatase 2A binding; microtubule binding; protein complex binding; apolipoprotein binding; SH3 domain binding; protein kinase binding

Biological Process: apoptosis; axon extension; positive regulation of axon extension; positive regulation of microtubule polymerization; axon cargo transport; neuron migration; female pregnancy; microtubule cytoskeleton organization and biogenesis; adult walking behavior; mitochondrion transport along microtubule; microtubule-based process; response to organic substance; neuron differentiation; axonogenesis; negative regulation of intracellular transport; regulation of autophagy; brain development; induction of apoptosis by oxidative stress; response to nutrient