Rat Microtubule-associated protein RP/EB family member 1 ELISA Kit | Mapre1 elisa kit

Rat Microtubule-associated protein RP/EB family member 1 ELISA Kit

Cat. Num. AAA2880060

• Gene Names: Mapre1; Eb1
• Reactivity: Rat
• Synonyms: Microtubule-associated protein RP/EB family member 1; Rat Microtubule-associated protein RP/EB family member 1 ELISA Kit; APC-binding protein EB1; End-binding protein 1; EB1; Mapre1; Mapre1 elisa kit

• Reactivity: Rat
• Specificity: Recombinant and natural Rat Microtubule-associated protein RP/EB family member 1
• Sequence Length: 268

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.156-10 ng/mL
• Sensitivity: < 0.091 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=4.5%
• Inter-assay Precision: Inter-Assay CV: <=8.9%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Mapre1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 78097100
• NCBI GeneID: 114764
• NCBI Accession #: NP_612518.2
• NCBI GenBank Nucleotide #: NM_138509.3
• UniProt Accession #: Q66HR2
• Molecular Weight: 30,004 Da
• NCBI Official Full Name: microtubule-associated protein RP/EB family member 1
• NCBI Official Synonym Full Names: microtubule-associated protein, RP/EB family, member 1
• NCBI Official Symbol: Mapre1
• NCBI Official Synonym Symbols: Eb1
• NCBI Protein Information: microtubule-associated protein RP/EB family member 1
• UniProt Protein Name: Microtubule-associated protein RP/EB family member 1
• Protein Family: Microtubule-associated protein RP/EB family
• UniProt Gene Name: Mapre1
• UniProt Synonym Gene Names: EB1
• UniProt Entry Name: MARE1_RAT

NCBI Description

expressed specifically at growing microtubule distal tips [RGD, Feb 2006]

Uniprot Description

EB1: a microtubule-associated protein of the RP/EB family. Binds to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. Localizes to microtubules, especially the growing ends, in interphase cells. Associates with the centrosomes and spindle microtubules during mitosis. Also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. May be involved in the regulation of microtubule structures and chromosome stability.

Protein type: Cytoskeletal; Cell cycle regulation; Microtubule-binding; Motility/polarity/chemotaxis

Cellular Component: cell projection; cell projection membrane; centrosome; cortical microtubule cytoskeleton; cytoplasm; cytoplasmic microtubule; Golgi apparatus; microtubule; microtubule cytoskeleton; spindle

Molecular Function: microtubule binding; microtubule plus-end binding

Biological Process: cell division; mitosis; negative regulation of microtubule polymerization