Rat High mobility group protein HMG-I/HMG-Y ELISA Kit | Hmga1 elisa kit

Rat High mobility group protein HMG-I/HMG-Y ELISA Kit

Cat. Num. AAA2881765

• Gene Names: Hmga1; Hmgi; Hmgiy
• Reactivity: Rat
• Synonyms: High mobility group protein HMG-I/HMG-Y; Rat High mobility group protein HMG-I/HMG-Y ELISA Kit; HMG-I(Y); High mobility group AT-hook protein 1; High mobility group protein A1; Hmga1; Hmga1 elisa kit

• Reactivity: Rat
• Specificity: Recombinant and natural Rat High mobility group protein HMG-I/HMG-Y,HMG-I(Y)
• Sequence Length: 107

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.156-10 ng/mL
• Sensitivity: <0.075 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=4.5%
• Inter-assay Precision: Inter-Assay CV: <=8.0%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Hmga1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 21326451
• NCBI GeneID: 117062
• NCBI Accession #: NP_647543.1
• NCBI GenBank Nucleotide #: NM_139327.1
• UniProt Accession #: Q8K585; Q8K1F5
• Molecular Weight: 10,709 Da
• NCBI Official Full Name: high mobility group protein HMG-I/HMG-Y
• NCBI Official Synonym Full Names: high mobility group AT-hook 1
• NCBI Official Symbol: Hmga1
• NCBI Official Synonym Symbols: Hmgi; Hmgiy
• NCBI Protein Information: high mobility group protein HMG-I/HMG-Y
• UniProt Protein Name: High mobility group protein HMG-I/HMG-Y
• Protein Family: High mobility group protein
• UniProt Gene Name: Hmga1
• UniProt Synonym Gene Names: HMG-I(Y); High mobility group protein A1
• UniProt Entry Name: HMGA1_RAT

NCBI Description

expressed in metastatic prostate tumors; may be associated with transformation [RGD, Feb 2006]

Uniprot Description

HMGA1: HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51B. Belongs to the HMGA family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Cellular Component: focal adhesion; nucleus

Molecular Function: 5'-deoxyribose-5-phosphate lyase activity; DNA binding; DNA-(apurinic or apyrimidinic site) lyase activity; enzyme binding; ligand-dependent nuclear receptor transcription coactivator activity; peroxisome proliferator activated receptor binding; retinoic acid receptor binding; retinoid X receptor binding; transcription factor activity; transcription factor binding

Biological Process: base-excision repair; negative regulation of cell proliferation; negative regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; regulation of transcription, DNA-dependent; response to virus; spermatogenesis; transcription, DNA-dependent