Human Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) RTU ELISA Kit | MAPRE1 rtu elisa kit

Human Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) ELISA Kit

Cat. Num. AAA4500907

• Gene Names: MAPRE1; EB1
• Reactivity: Human
• Synonyms: Microtubule Associated Protein RP/EB Family; Member 1 (MAPRE1); Human Microtubule Associated Protein RP/EB Family; Member 1 (MAPRE1) ELISA Kit; EB1; Adenomatous Polyposis Coli-Binding protein; APC-binding protein EB1; End-binding protein 1; MAPRE1 rtu elisa kit

• Reactivity: Human
• Sequence Length: 268

• Assay Type: Sandwich
• Samples: Tissue Homogenates, Cell Lysates or Other Biological Fluids.
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.036ng/mL
• RTU ELISA Kit's Advantage: The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.
• Preparation and Storage: For unopened kits: All the reagents should be kept at 4 degree C upon receipt.; For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAPRE1 rtu elisa kit

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to MAPRE1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to MAPRE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MAPRE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MAPRE1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6912494
• NCBI GeneID: 22919
• NCBI Accession #: NP_036457.1
• NCBI GenBank Nucleotide #: NM_012325.2
• UniProt Accession #: Q15691; Q3KQS8; B2R6I7; E1P5M8
• Molecular Weight: 29,999 Da
• NCBI Official Full Name: microtubule-associated protein RP/EB family member 1
• NCBI Official Synonym Full Names: microtubule associated protein RP/EB family member 1
• NCBI Official Symbol: MAPRE1
• NCBI Official Synonym Symbols: EB1
• NCBI Protein Information: microtubule-associated protein RP/EB family member 1
• UniProt Protein Name: Microtubule-associated protein RP/EB family member 1
• Protein Family: Microtubule-associated protein RP/EB family
• UniProt Gene Name: MAPRE1
• UniProt Synonym Gene Names: EB1

NCBI Description

The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq, Jul 2008]

Uniprot Description

Plus-end tracking protein (+TIP) that binds to the plus-end of microtubules and regulates the dynamics of the microtubule cytoskeleton (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:28726242, PubMed:28814570). Promotes cytoplasmic microtubule nucleation and elongation (PubMed:12388762, PubMed:16109370, PubMed:19632184, PubMed:21646404, PubMed:28726242, PubMed:28814570). May be involved in spindle function by stabilizing microtubules and anchoring them at centrosomes (PubMed:12388762). Also acts as a regulator of minus-end microtubule organization: interacts with the complex formed by AKAP9 and PDE4DIP, leading to recruit CAMSAP2 to the Golgi apparatus, thereby tethering non-centrosomal minus-end microtubules to the Golgi, an important step for polarized cell movement (PubMed:28814570). Promotes elongation of CAMSAP2-decorated microtubule stretches on the minus-end of microtubules (PubMed:28814570). Acts as a regulator of autophagosome transport via interaction with CAMSAP2 (PubMed:28726242). May play a role in cell migration ().