Rabbit anti-Mouse SEC14L2 Polyclonal Antibody | anti-SEC14L2 antibody

SEC14L2, NT (SEC14L2, C22orf6, KIAA1186, KIAA1658, SEC14-like protein 2, Alpha-tocopherol-associated protein, Squalene transfer protein, Supernatant protein factor) (Biotin)

Cat. Num. AAA6345725

• Gene Names: SEC14L2; SPF; TAP; TAP1; C22orf6
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SEC14L2; Polyclonal Antibody; NT (SEC14L2; C22orf6; KIAA1186; KIAA1658; SEC14-like protein 2; Alpha-tocopherol-associated protein; Squalene transfer protein; Supernatant protein factor) (Biotin); Supernatant protein factor; anti-SEC14L2 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 403

• Applicable Applications for anti-SEC14L2 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: SEC14L2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 40-69 amino acids from the N-terminal region of human SEC14L2.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(SEC14L2 Antibody (N-term) western blot analysis in mouse liver tissue lysates (35ug/lane).This demonstrates the SEC14L2 antibody detected the SEC14L2 protein (arrow).)

Related Product Information for anti-SEC14L2 antibody

This gene encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p, alpha-tocopherol transfer protein, and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.

Product Categories/Family for anti-SEC14L2 antibody

Antibodies; KIAA; orf

References

Guey, L.T., et al. Eur. Urol. 57(2):283-292(2010). Wang, X., et al. Cancer Invest. 27(10):971-977(2009). Hosgood, H.D. III, et al. Respir Med 103(12):1866-1870(2009). Johnykutty, S., et al. Mod. Pathol. 22(6):770-775(2009). Wright, M.E., et al. Cancer Res. 69(4):1429-1438(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 7110715
• NCBI GeneID: 23541
• NCBI Accession #: NP_036561.1; NP_203740.1
• NCBI GenBank Nucleotide #: NP_036561.1
• UniProt Accession #: O76054
• NCBI Official Full Name: SEC14-like protein 2 isoform 1
• NCBI Official Synonym Full Names: SEC14 like lipid binding 2
• NCBI Official Symbol: SEC14L2
• NCBI Official Synonym Symbols: SPF; TAP; TAP1; C22orf6
• NCBI Protein Information: SEC14-like protein 2
• UniProt Protein Name: SEC14-like protein 2
• Protein Family: SEC14-like protein
• UniProt Gene Name: SEC14L2
• UniProt Synonym Gene Names: C22orf6; KIAA1186; KIAA1658; TAP; hTAP; SPF
• UniProt Entry Name: S14L2_HUMAN

NCBI Description

This gene encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p, alpha-tocopherol transfer protein, and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008]

Uniprot Description

SEC14L2: Carrier protein. Binds to some hydrophobic molecules and promotes their transfer between the different cellular sites. Binds with high affinity to alpha-tocopherol. Also binds with a weaker affinity to other tocopherols and to tocotrienols. May have a transcriptional activatory activity via its association with alpha-tocopherol. Probably recognizes and binds some squalene structure, suggesting that it may regulate cholesterol biosynthesis by increasing the transfer of squalene to a metabolic active pool in the cell. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid-binding

Chromosomal Location of Human Ortholog: 22q12.2

Cellular Component: cytoplasm; integral to membrane; nucleus

Molecular Function: transporter activity; phospholipid binding; vitamin E binding

Biological Process: transcription, DNA-dependent; transport; regulation of cholesterol biosynthetic process; positive regulation of transcription, DNA-dependent