Western Blot (WB)
(Western blot analysis of SARP3 expression in mouse pancreas (A) whole cell lysates.)
Rabbit SARP3 Polyclonal Antibody | anti-SARP3 antibody
Anti-SARP3 Antibody
Gene Names
SFRP5; SARP3
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry
Purity
Purified by Immunogen Affinity Chromatography
Synonyms
SARP3; Polyclonal Antibody; Anti-SARP3 Antibody; FRP1B; Secreted frizzled-related protein 5; sFRP-5; Frizzled-related protein 1b; FRP-1b; Secreted apoptosis-related protein 3; SARP-3; anti-SARP3 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
Recognizes endogenous levels of SARP3 protein.
Purity/Purification
Purified by Immunogen Affinity Chromatography
Form/Format
Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
Applicable Applications for anti-SARP3 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
Application Notes
WB: 1:500-1:2000
IF/ICC: 1:50-1:200
IF/ICC: 1:50-1:200
Immunogen
Recombinant fusion protein of human SARP3. The exact sequence is proprietary.
Preparation and Storage
Upon delivery aliquot and store at -20 degree C for one year. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of SARP3 expression in mouse pancreas (A) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of SARP3 expression in mouse pancreas (A) whole cell lysates.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of SARP3 staining in mouse pancreas tissues. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(Immunofluorescent analysis of SARP3 staining in mouse pancreas tissues. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 degree C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.)
Related Product Information for anti-SARP3 antibody
Rabbit polyclonal antibody to SARP3
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,563 Da
NCBI Official Full Name
secreted frizzled-related protein 5
NCBI Official Synonym Full Names
secreted frizzled-related protein 5
NCBI Official Symbol
SFRP5
NCBI Official Synonym Symbols
SARP3
NCBI Protein Information
secreted frizzled-related protein 5; FRP-1b; SARP-3; sFRP-5; frizzled-related protein 1b; secreted apoptosis related protein 3; secreted apoptosis-related protein 3
UniProt Protein Name
Secreted frizzled-related protein 5
UniProt Gene Name
SFRP5
UniProt Synonym Gene Names
FRP1B; SARP3; sFRP-5; FRP-1b; SARP-3
UniProt Entry Name
SFRP5_HUMAN
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Product Notes
The SARP3 sfrp5 (Catalog #AAA8308071) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-SARP3 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SARP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC). WB: 1:500-1:2000 IF/ICC: 1:50-1:200. Researchers should empirically determine the suitability of the SARP3 sfrp5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SARP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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