Rat Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) ELISA Kit | MAPRE1 elisa kit

Rat Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) ELISA Kit

Cat. Num. AAA2098093

• Gene Names: MAPRE1; EB1
• Reactivity: Rat
• Synonyms: Microtubule Associated Protein RP/EB Family; Member 1 (MAPRE1); Rat Microtubule Associated Protein RP/EB Family; Member 1 (MAPRE1) ELISA Kit; EB1; Adenomatous Polyposis Coli-Binding protein; APC-binding protein EB1; End-binding protein 1; MAPRE1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1).; No significant cross-reactivity or interference between Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) and analogues was observed.
• Sequence Length: 268

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.122ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for MAPRE1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Microtubule Associated Protein RP/EB Family, Member 1 (MAPRE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for MAPRE1 elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 6912494
• NCBI GeneID: 22919
• NCBI Accession #: NP_036457.1
• NCBI GenBank Nucleotide #: NM_012325.2
• UniProt Accession #: Q15691; Q3KQS8; B2R6I7; E1P5M8
• Molecular Weight: 29,999 Da
• NCBI Official Full Name: microtubule-associated protein RP/EB family member 1
• NCBI Official Synonym Full Names: microtubule associated protein RP/EB family member 1
• NCBI Official Symbol: MAPRE1
• NCBI Official Synonym Symbols: EB1
• NCBI Protein Information: microtubule-associated protein RP/EB family member 1
• UniProt Protein Name: Microtubule-associated protein RP/EB family member 1
• Protein Family: Microtubule-associated protein RP/EB family
• UniProt Gene Name: MAPRE1
• UniProt Synonym Gene Names: EB1

NCBI Description

The protein encoded by this gene was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family. [provided by RefSeq, Jul 2008]

Uniprot Description

EB1: a microtubule-associated protein of the RP/EB family. Binds to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. Localizes to microtubules, especially the growing ends, in interphase cells. Associates with the centrosomes and spindle microtubules during mitosis. Also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. May be involved in the regulation of microtubule structures and chromosome stability.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 20q11.21

Cellular Component: cell projection membrane; centrosome; cortical microtubule cytoskeleton; cytoplasmic microtubule; cytosol; Golgi apparatus; microtubule; spindle

Molecular Function: cadherin binding; identical protein binding; microtubule plus-end binding; protein binding; protein C-terminus binding; protein kinase binding; RNA binding

Biological Process: cell division; cell proliferation; G2/M transition of mitotic cell cycle; negative regulation of microtubule binding; negative regulation of microtubule polymerization; positive regulation of cell migration; protein localization; regulation of G2/M transition of mitotic cell cycle; sister chromatid cohesion