Bovine Cartilage oligomeric matrix protein ELISA Kit | COMP elisa kit

Bovine Cartilage oligomeric matrix protein ELISA Kit

Cat. Num. AAA2884547

• Reactivity: Bovine
• Synonyms: Cartilage oligomeric matrix protein; Bovine Cartilage oligomeric matrix protein ELISA Kit; COMP; COMP elisa kit

• Reactivity: Bovine
• Specificity: Recombinant and natural Bovine Cartilage oligomeric matrix protein,COMP
• Sequence Length: 756

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Detection Range: 0.312-20 ng/mL
• Sensitivity: <0.078 ng/mL
• Intra-assay Precision: Intra-Assay CV: <=5.2%
• Inter-assay Precision: Inter-Assay CV: <=8.9%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COMP elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 145559457
• NCBI GeneID: 281088
• UniProt Accession #: P35445
• Molecular Weight: 82,362 Da
• NCBI Official Full Name: Cartilage oligomeric matrix protein
• NCBI Official Symbol: COMP
• NCBI Protein Information: cartilage oligomeric matrix protein
• UniProt Protein Name: Cartilage oligomeric matrix protein
• Protein Family: Cartilage oligomeric matrix protein
• UniProt Gene Name: COMP
• UniProt Synonym Gene Names: COMP
• UniProt Entry Name: COMP_BOVIN

Uniprot Description

May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7 ().