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SDS-Page

Lactoferrin (LTF) Recombinant Protein | LTF recombinant protein

Recombinant Lactoferrin (LTF)

Gene Names
Ltf; Lf; Csp82; Ms10r; MMS10R
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Lactoferrin (LTF); Recombinant Lactoferrin (LTF); LTF recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-C LAEKAGNVAF LKDSTVLQNT DGKNTEEWAR NLKLKDFELL CLDDTRKPVT EAKNCHLAIA PNHAVVSRTD KVEVLQQVLL DQQVQFGRNG QRCPGEFCLF QSKTKNLLFN DNTECLAKIP GKTTSEKYLGKEYVIATERL KQCSSSPLLE ACAFLTQ
Sequence Length
707
Applicable Applications for LTF recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Mus musculus (Mouse)
Expression System
Prokaryotic expression
Residues
Cys550~Gln707 (Accession # P08071) with two N-terminal Tags, His-tag and GST-tag
Subcellular Location
Secreted
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
6.3
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
49.7kDa
NCBI Official Full Name
lactotransferrin
NCBI Official Synonym Full Names
lactotransferrin
NCBI Official Symbol
Ltf
NCBI Official Synonym Symbols
Lf; Csp82; Ms10r; MMS10R
NCBI Protein Information
lactotransferrin; N lobe; lactoferrin; minisatellite 10r detected by probe MMS10
UniProt Protein Name
Lactotransferrin
Protein Family
UniProt Gene Name
Ltf
UniProt Synonym Gene Names
Lactoferrin
UniProt Entry Name
TRFL_MOUSE

Uniprot Description

LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: Secreted; EC 3.4.21.-; Protease; Secreted, signal peptide

Cellular Component: extracellular space; specific granule; cytoplasm; extracellular region; nucleus; secretory granule

Molecular Function: heparin binding; peptidase activity; protein serine/threonine kinase activator activity; hydrolase activity; serine-type peptidase activity; iron ion binding; metal ion binding

Biological Process: negative regulation of lipopolysaccharide-mediated signaling pathway; ossification; positive regulation of I-kappaB kinase/NF-kappaB cascade; immune system process; positive regulation of toll-like receptor 4 signaling pathway; antifungal humoral response; proteolysis; activation of NF-kappaB transcription factor; regulation of cytokine production; positive regulation of osteoblast differentiation; iron ion homeostasis; negative regulation of viral genome replication; innate immune response in mucosa; transport; positive regulation of osteoblast proliferation; antibacterial humoral response; regulation of tumor necrosis factor production; ion transport; negative regulation of ATPase activity; negative regulation of viral reproduction

Research Articles on LTF

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Product Notes

The LTF ltf (Catalog #AAA2010791) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Lactoferrin (LTF) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the LTF ltf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below. MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -C LAEKAGNVAF LKDSTVLQNT DGKNTEEWAR NLKLKDFELL CLDDTRKPVT EAKNCHLAIA PNHAVVSRTD KVEVLQQVLL DQQVQFGRNG QRCPGEFCLF QSKTKNLLFN DNTECLAKIP GKTTSEKYLG KEYVIATERL KQCSSSPLLE ACAFLTQ. It is sometimes possible for the material contained within the vial of "Lactoferrin (LTF), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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