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Western Blot (WB) (Western Blot: Sample: Mouse Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse LTF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Rabbit anti-Mouse Lactoferrin (LTF) Polyclonal Antibody | anti-LTF antibody

Polyclonal Antibody to Lactoferrin (LTF)

Gene Names
Ltf; Lf; Csp82; Ms10r; MMS10R
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Lactoferrin (LTF); Polyclonal Antibody; Polyclonal Antibody to Lactoferrin (LTF); anti-LTF antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against LTF. It has been selected for its ability to recognize LTF in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-C LAEKAGNVAF LKDSTVLQNT DGKNTEEWAR NLKLKDFELL CLDDTRKPVT EAKNCHLAIA PNHAVVSRTD KVEVLQQVLL DQQVQFGRNG QRCPGEFCLF QSKTKNLLFN DNTECLAKIP GKTTSEKYLG KEYVIATERL KQCSSSPLLE ACAFLTQ
Sequence Length
475
Applicable Applications for anti-LTF antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant LTF (Cys550~Gln707) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2047281
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Mouse Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse LTF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB) (Western Blot: Sample: Mouse Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse LTF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P. Samples: Mouse Tissue))

Immunohistochemistry (IHC) (DAB staining on IHC-P. Samples: Mouse Tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
77,838 Da
NCBI Official Full Name
lactotransferrin
NCBI Official Synonym Full Names
lactotransferrin
NCBI Official Symbol
Ltf
NCBI Official Synonym Symbols
Lf; Csp82; Ms10r; MMS10R
NCBI Protein Information
lactotransferrin
UniProt Protein Name
Lactotransferrin
Protein Family
UniProt Gene Name
Ltf
UniProt Synonym Gene Names
Lactoferrin

Uniprot Description

LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: EC 3.4.21.-; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9 F2|9 60.79 cM

Cellular Component: cell surface; cytoplasm; extracellular exosome; extracellular region; extracellular space; nucleus; other organism cell membrane; protein complex; secretory granule; specific granule

Molecular Function: cysteine-type endopeptidase inhibitor activity; heparin binding; hydrolase activity; iron ion binding; lipopolysaccharide binding; metal ion binding; peptidase activity; protein serine/threonine kinase activator activity; serine-type endopeptidase activity; serine-type peptidase activity

Biological Process: antibacterial humoral response; antifungal humoral response; antimicrobial humoral immune response mediated by antimicrobial peptide; defense response to Gram-negative bacterium; immune system process; innate immune response in mucosa; ion transport; iron ion homeostasis; killing of cells of other organism; membrane disruption in other organism; negative regulation by host of viral process; negative regulation of ATPase activity; negative regulation of cysteine-type endopeptidase activity; negative regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of single-species biofilm formation in or on host organism; negative regulation of viral genome replication; negative regulation of viral process; ossification; positive regulation of chondrocyte proliferation; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of NF-kappaB transcription factor activity; positive regulation of osteoblast differentiation; positive regulation of osteoblast proliferation; positive regulation of protein serine/threonine kinase activity; positive regulation of toll-like receptor 4 signaling pathway; proteolysis; regulation of cytokine production; regulation of tumor necrosis factor production; transport

Research Articles on LTF

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Product Notes

The LTF ltf (Catalog #AAA2004703) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Lactoferrin (LTF) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Lactoferrin (LTF) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the LTF ltf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -C LAEKAGNVAF LKDSTVLQNT DGKNTEEWAR NLKLKDFELL CLDDTRKPVT EAKNCHLAIA PNHAVVSRTD KVEVLQQVLL DQQVQFGRNG QRCPGEFCLF QSKTKNLLFN DNTECLAKIP GKTTSEKYLG KEYVIATERL KQCSSSPLLE ACAFLTQ. It is sometimes possible for the material contained within the vial of "Lactoferrin (LTF), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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