MICA recombinant protein

Recombinant Human MICA Protein (His tag)

Cat. Num. AAA2545525

• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: MICA; Recombinant Human MICA Protein (His tag); DAMA-345G11.2; FLJ36918; FLJ60820; MGC111087; MGC21250; MIC-A; PERB11.1; MICA recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 383

• Application Notes: The recombinant human MICA consists of 296 amino acids and has a predicted molecular mass of 34 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh MICA is approximately 55-65 kDa due to glycosylation.
• Predicted N Terminal: Glu 24
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for MICA recombinant protein

Background: MHC class I chain-related molecules A (MICA) is one of the genes in the HLA class I region, which belongs to MHC class I family. It is the member of the non-classical class I family that displays the greatest degree of polymorphism. The MICA protein product is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtype s of T cells. The Natural killer group 2D (NKG2D), a C-type lectin-like activating immunoreceptor, is a receptor of MICA, which was detected on most gammadelta T cells, CD8+ alphabeta T cells, and natural killer (NK) cells. Effector cells from all these subsets could be stimulated by ligation of NKG2D. Engagement of NKG2D activated cytolytic responses of gammadelta T cells and NK cells against transfectants and epithelial tumor cells expressing MICA. The MICA system is a novel, avidin-free immunohistochemical detection system that provides a significant increase in sensitivity compared to traditional immunodetection systems.

Description: A DNA sequence encoding the human MICA (AAH16929.1) extracellular domain (Met 1-Gln 308) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 16877353
• NCBI GeneID: 4276
• Molecular Weight: 31,577 Da
• NCBI Official Full Name: MHC class I polypeptide-related sequence A
• UniProt Protein Name: MHC class I polypeptide-related sequence A
• Protein Family: MICAL-like protein
• UniProt Gene Name: MICA
• UniProt Synonym Gene Names: MIC-A

Uniprot Description

MICA: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T- cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. Anti-MICA antibodies and ligand shedding are involved in the progression of monoclonal gammopathy of undetermined significance (MGUS)to multiple myeloma. Genetic variations in MICA may be a cause of susceptibility to psoriasis type 1 (PSORS1). Psoriasis is a common, chronic inflammatory disease of the skin with multifactorial etiology. It is characterized by red, scaly plaques usually found on the scalp, elbows and knees. These lesions are caused by abnormal keratinocyte proliferation and infiltration of inflammatory cells into the dermis and epidermis. Genetic variation in MICA is a cause of susceptibility to psoriatic arthritis (PSORAS). PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). Belongs to the MHC class I family. MIC subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: cell surface; extracellular space; integral to plasma membrane; plasma membrane

Molecular Function: antigen binding; beta-2-microglobulin binding; natural killer cell lectin-like receptor binding; protein binding

Biological Process: antigen processing and presentation; defense response to bacterium; defense response to virus; gamma-delta T cell activation; immune response to tumor cell; natural killer cell mediated cytotoxicity; negative regulation of natural killer cell activation; negative regulation of natural killer cell mediated cytotoxicity; regulation of immune response; response to DNA damage stimulus; response to heat; T cell mediated cytotoxicity

Disease: Psoriasis 1, Susceptibility To; Psoriatic Arthritis, Susceptibility To