Lactotransferrin / LTF Recombinant Protein | LTF recombinant protein

Recombinant Mouse Lactotransferrin / LTF Protein (His tag)

Cat. Num. AAA2546805

• Gene Names: Ltf; Lf; Csp82; Ms10r; MMS10R
• Purity: > 92 % as determined by SDS-PAGE
• Synonyms: Lactotransferrin / LTF; Recombinant Mouse Lactotransferrin / LTF Protein (His tag); Csp82; Lf; MMS10R; Ms10r; LTF recombinant protein

• Host: Human Cells
• Purity/Purification: > 92 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 707

• Application Notes: The secreted recombinant mouse LTF comprises 699 amino acids and has a calculated molecular mass of 77.2 kDa. The apparent molecular mass of the recombinant protein is approximately 70 kDa in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Ala 28
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for LTF recombinant protein

Background: Lactotransferrin, also known as Lactoferrin, Talalactoferrin and LTF, is a secreted protein which belongs to the transferrin family. Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. Lactotransferrin has antimicrobial activity which depends on the extracellular cation concentration. Lactoferroxins A, B and C have opioid antagonist activity. Lactoferroxin A shows preference for mu-receptors, while lactoferroxin B and lactoferroxin C have somewhat higher degrees of preference for kappa-receptors than for mu-receptors. Lactoferrin / LTF is a globular glycoprotein that is widely represented in various secretory fluids, such as milk, saliva, tears, and nasal secretions. Lactoferrin / LTF is also present in secondary granules of PMN and is secreted by some acinar cells. Lactoferrin / LTF can be purified from milk or produced recombinantly. Human colostrum has the highest concentration, followed by human milk, then cow milk. Lactoferrin / LTF is one of the components of the immune system of the body; it has antimicrobial activity (bacteriocide, fungicide) and is part of the innate defense, mainly at mucoses. In particular, lactoferrin provides antibacterial activity to human infants. Lactoferrin interacts with DNA and RNA, polysaccharides and heparin, and shows some of its biological functions in complexes with these ligands.

Description: A DNA sequence encoding the mouse LTF (AAH06904.1) (Met 1-Gln 707) was expressed, with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 13905216
• NCBI GeneID: 17002
• Molecular Weight: 77,838 Da
• NCBI Official Full Name: Lactotransferrin
• NCBI Official Synonym Full Names: lactotransferrin
• NCBI Official Symbol: Ltf
• NCBI Official Synonym Symbols: Lf; Csp82; Ms10r; MMS10R
• NCBI Protein Information: lactotransferrin
• UniProt Protein Name: Lactotransferrin
• Protein Family: Lactotransferrin
• UniProt Gene Name: Ltf
• UniProt Synonym Gene Names: Lactoferrin

Uniprot Description

LTF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. Belongs to the transferrin family. 2 isoforms of the human protein are produced by alternative promoter.

Protein type: EC 3.4.21.-; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9 F2|9 60.79 cM

Cellular Component: cell surface; cytoplasm; extracellular space; nucleus; protein complex; secretory granule; specific granule

Molecular Function: heparin binding; iron ion binding; peptidase activity; protein serine/threonine kinase activator activity

Biological Process: activation of NF-kappaB transcription factor; antibacterial humoral response; antifungal humoral response; innate immune response in mucosa; negative regulation of ATPase activity; negative regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of viral genome replication; negative regulation of viral reproduction; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of osteoblast differentiation; positive regulation of osteoblast proliferation; positive regulation of toll-like receptor 4 signaling pathway; regulation of cytokine production; regulation of tumor necrosis factor production