Lactoferrin (LTF) Recombinant Protein | LTF recombinant protein

Recombinant Lactoferrin (LTF)

Cat. Num. AAA2010337

• Gene Names: LTF; PLF
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Lactoferrin (LTF); Recombinant Lactoferrin (LTF); LTF recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-VRWCVI STAEYSKCRQ WQSKIRRTNP MFCIRRASPT DCIRAIAAKR ADAVTLDGGL VFEADQYKLR PVAAEIYGTE ENPQTYYYAV AVVKKGFNFQ LNQLQGRKSC HTGLGRSAGW NIPIGLLRRF LDWAGPPEPL QKAVAKFFSQ SCVPCADGNA YPNLCQLCIG KGKDKCACSS QEPYFGYSGA FNCLHKGIGD VAFVKESTVF ENLPQKADRD KYELLCPDNT RKPVEAFREC HLARVPSHAV VARSVNGKEN SIWELLYQSQ KKFGKSNPQE FQLFGSPGQQ KDLLFRDATI GFLKIPSKID SKLYLGLPYLTAIQGLRE
• Sequence Length: 686

• Applicable Applications for LTF recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Sus scrofa; Porcine (Pig)
• Expression System: Prokaryotic expression
• Residues: Val25~Glu348 (Accession # P14632) with two N-terminal Tags, His-tag and GST-tag
• Subcellular Location: Secreted
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 8.7
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 47523782
• NCBI GeneID: 397649
• NCBI Accession #: NP_999527.1
• NCBI GenBank Nucleotide #: NM_214362.1
• UniProt Accession #: P14632; Q29557
• Molecular Weight: 68.0kDa
• NCBI Official Full Name: lactotransferrin
• NCBI Official Symbol: LTF
• NCBI Official Synonym Symbols: PLF
• NCBI Protein Information: lactotransferrin; lactoferrin
• UniProt Protein Name: Lactotransferrin
• Protein Family: Lactotransferrin
• UniProt Gene Name: LTF
• UniProt Synonym Gene Names: Lactoferrin
• UniProt Entry Name: TRFL_PIG

Uniprot Description

Function: Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate.Lactotransferrin is a major iron-binding and multifunctional protein found in exocrine fluids such as breast milk and mucosal secretions. Has antimicrobial activity. Antimicrobial properties may include bacteriostasis, which is related to its ability to sequester free iron and thus inhibit microbial growth, as well as direct bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane. May have anabolic, differentiating and anti-apoptotic effects on osteoblasts and may also inhibit osteoclastogenesis, possibly playing a role in the regulation of bone growth. May interfere with the lipopolysaccharide (LPS)-stimulated TLR4 signaling

By similarity.The lactotransferrin transferrin-like domain 1 functions as a serine protease of the peptidase S60 family that cuts arginine rich regions. This function contributes to the antimicrobial activity

By similarity.

Catalytic activity: Preferential at -Arg-Ser-Arg-Arg-|- and -Arg-Arg-Ser-Arg-|-, and of Z-Phe-Arg-|-aminomethylcoumarin.

Subunit structure: Monomer. Found in a complex with LTF, CLU, EPPIN and SEMG1

By similarity.

Subcellular location: Secreted. Cytoplasmic granule

By similarity. Note: Secreted into most exocrine fluids by various endothelial cells. Stored in the secondary granules of neutrophils

By similarity.

Sequence similarities: Belongs to the transferrin family.Contains 2 transferrin-like domains.