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Western Blot (WB) (Western blot analysis of lysates from K562 and A549 cells, using DAK Antibody. The lane on the right is blocked with the synthesized peptide.)

Rabbit DAK Polyclonal Antibody | anti-DAK antibody

DAK Antibody

Gene Names
TKFC; DAK; NET45
Reactivity
Human, Mouse, Rat
Applications
Western Blot, ELISA
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Synonyms
DAK; Polyclonal Antibody; DAK Antibody; DHA kinase; Dihydroxyacetone kinase; Glycerone kinase; anti-DAK antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Specificity
DAK Antibody detects endogenous levels of total DAK protein.
Purity/Purification
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Form/Format
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1 mg/ml (varies by lot)
Sequence Length
534
Applicable Applications for anti-DAK antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:500~1:1000
ELISA: 1:20000
Target Modification
Unmodified/Total
Immunogen
The antiserum was produced against synthesized peptide derived from human DAK.
Immunogen Range
91-140
Preparation and Storage
Stable at -20 degree C for at least 1 year.

Western Blot (WB)

(Western blot analysis of lysates from K562 and A549 cells, using DAK Antibody. The lane on the right is blocked with the synthesized peptide.)

Western Blot (WB) (Western blot analysis of lysates from K562 and A549 cells, using DAK Antibody. The lane on the right is blocked with the synthesized peptide.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
58 kDa
NCBI Official Full Name
triokinase/FMN cyclase isoform b
NCBI Official Synonym Full Names
triokinase and FMN cyclase
NCBI Official Symbol
TKFC
NCBI Official Synonym Symbols
DAK; NET45
NCBI Protein Information
triokinase/FMN cyclase
UniProt Protein Name
Triokinase/FMN cyclase
Protein Family
UniProt Gene Name
TKFC
UniProt Synonym Gene Names
DHA kinase

NCBI Description

This gene is a member of the family of dihydroxyacetone kinases, which have a protein structure distinct from other kinases. The product of this gene phosphorylates dihydroxyacetone, and also catalyzes the formation of riboflavin 4',5'-phosphate (aka cyclin FMN) from FAD. Several alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2017]

Uniprot Description

Catalyzes both the phosphorylation of dihydroxyacetone and of glyceraldehyde, and the splitting of ribonucleoside diphosphate-X compounds among which FAD is the best substrate. Represses IFIH1-mediated cellular antiviral response (PubMed:17600090).

Research Articles on DAK

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Product Notes

The DAK tkfc (Catalog #AAA9505277) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DAK Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's DAK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:500~1:1000 ELISA: 1:20000. Researchers should empirically determine the suitability of the DAK tkfc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DAK, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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