Human Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP) ELISA Kit | PDCD6IP elisa kit

Human Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP) ELISA Kit

Cat. Num. AAA450794

• Gene Names: PDCD6IP; AIP1; ALIX; HP95; DRIP4
• Reactivity: Human
• Synonyms: Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP); Human Programmed Cell Death Protein 6 Interacting Protein (PDCD6IP) ELISA Kit; ASD3; MYHC; MYHCA; Alpha-MHC; Cardiomyopathy; Hypertrophic 1; PDCD6IP elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PCT. No significant cross-reactivity or interference between PCT and analogues was observed.
• Sequence Length: 873

• Samples: Human Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates Or Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.2-2000pg/mL
• Sensitivity: 12.6pg/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PCT were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PCT were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100.
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PDCD6IP elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PCT in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PCT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to PCT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PCT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PCT in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 241982780
• NCBI GeneID: 10015
• NCBI Accession #: NP_001155901.1
• NCBI GenBank Nucleotide #: NM_001162429.2
• UniProt Accession #: Q8WUM4; Q6NUS1; Q9BX86; Q9NUN0; Q9P2H2; Q9UKL5; C5MQH7; E9PFU1
• Molecular Weight: 30,453 Da
• NCBI Official Full Name: programmed cell death 6-interacting protein isoform 2
• NCBI Official Synonym Full Names: programmed cell death 6 interacting protein
• NCBI Official Symbol: PDCD6IP
• NCBI Official Synonym Symbols: AIP1; ALIX; HP95; DRIP4
• NCBI Protein Information: programmed cell death 6-interacting protein
• UniProt Protein Name: Programmed cell death 6-interacting protein
• Protein Family: Programmed cell death 6-interacting protein
• UniProt Gene Name: PDCD6IP
• UniProt Synonym Gene Names: AIP1; ALIX; KIAA1375; PDCD6-interacting protein

NCBI Description

This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]

Uniprot Description

Class E VPS protein involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome. Binds to the phospholipid lysobisphosphatidic acid (LBPA) which is abundant in MVBs internal membranes. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). Appears to be an adapter for a subset of ESCRT-III proteins, such as CHMP4, to function at distinct membranes. Required for completion of cytokinesis. Involved in HIV-1 virus budding. Can replace TSG101 it its role of supporting HIV-1 release; this function implies the interaction with CHMP4B. May play a role in the regulation of both apoptosis and cell proliferation. Regulates exosome biogenesis in concert with SDC1/4 and SDCBP (PubMed:22660413).