KLHL2 recombinant protein

KLHL2 Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8121717

• Purity: >85% as determined by SDS-PAGE
• Synonyms: KLHL2; KLHL2 Protein; Human; Recombinant (GST Tag); Human KLHL2 Protein (GST Tag); ABP-KELCH Protein; MAV Protein; MAYVEN Protein; kelch like family member 2; KLHL2 recombinant protein

• Host: E Coli
• Purity/Purification: >85% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 10% glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro306

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the mature form of human KLHL2 (AAH36468.1)(Met1-Pro306) was fused with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for KLHL2 recombinant protein

Background: KLHL2 (Kelch Like Family Member 2) is a Protein Coding gene. 3 alternatively spliced human isoforms have been reported. KLHL2 contains 1 BTB (POZ) domain and 6 Kelch repeats. It is widely expressed in the brain, esophagus, and other tissues. KLHL2 gene has been proposed to participate in intracellular protein transportation. KLHL2 is expected to have molecular functions such as transporter activity, actin-binding, and protein binding. KLHL2 localizes in various compartments such as actin cytoskeleton, cytoplasm, membrane, and nucleus. It may also play a role in organizing the actin cytoskeleton of the brain cells. Diseases associated with KLHL2 include Mixed Malaria and Inclusion Body Myopathy With Early-Onset Paget Disease Of Bone With Or Without Frontotemporal Dementia 2.

References

Minx P, et al. (2005) Generation and annotation of the DNA sequences of human chromosomes 2 and 4. Nature. 434:724-31. Ota T, et al. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. Nat Genet. 36:40-5. Jiang S, et al. (1999) Characterization of Mayven, a novel actin-binding protein predominantly expressed in brain. Mol Biol Cell. 10:2361-75.

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant human KLHL2 /GST chimera consists of 540 amino acids and has a predicted molecular mass of 62.2kDa. It migrates as an approximately 63kDa band in SDS-PAGE under reducing conditions.
• Protein Family: Kelch-like protein