ATP6V1F recombinant protein

ATP6V1F Protein, Human, Recombinant (GST Tag)

Cat. Num. AAA8121912

• Purity: >90% as determined by SDS-PAGE
• Synonyms: ATP6V1F; ATP6V1F Protein; Human; Recombinant (GST Tag); Human ATP6V1F Protein (GST Tag); ATP6S14 Protein; VATF Protein; Vma7 Protein; ATPase; H+ transporting; lysosomal 14kDa; V1 subunit F; ATP6V1F recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Arg119

• Species: Human
• Predicted N Terminal: Met
• Tag: N-GST
• Protein Construction: A DNA sequence encoding the human ATP6V1F (NP_004222.2)(Met1-Arg119) was expressed with the GST tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ATP6V1F recombinant protein

Background: ATP6V1F encodes a component of vacuolar ATPase mediating acidification. The cDNA and the genomic sequences of ATP6V1F were cloned successfully for the first time from the Giant Panda (Ailuropoda melanoleuca) using reverse transcription polymerase chain reaction and touchdown-polymerase chain reaction, respectively. Topology prediction showed that there is one protein kinase C phosphorylation site, two Casein kinase II phosphorylation sites, and one N-myristoylation site in the ATP6V1F protein. Up-regulated expression of mammary tumor 8kDa protein (MAT-8), complement component C1S (C1S), ferritin heavy chain (FTH1), peptidyl-prolyl cis-trans isomerase A (PPIA), RNA-binding protein regulatory subunit DJ-1 protein (DJ-1) and vacuolar ATP synthase subunit F (ATP6V1F) was determined in prostate carcinoma and confirmed by using quantitative real-time RT-PCR analyses.

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant human ATP6V1F/GST chimera consists of 350 amino acids and has a predicted molecular mass of 40.2kDa. It migrates as an approximately 40kDa band in SDS-PAGE under reducing conditions.