T Plastin/PLS3 Recombinant Protein | PLS3 recombinant protein

T Plastin/PLS3 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121798

• Gene Names: PLS3; BMND18; T-plastin
• Purity: >95% as determined by SDS-PAGE
• Synonyms: T Plastin/PLS3; T Plastin/PLS3 Protein; Human; Recombinant (His Tag); Human PLS3/Plastin 3 Protein (His Tag); BMND18 Protein; T-plastin Protein; plastin 3; PLS3 recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, 10% Glycerol, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gly102-Asn375

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human PLS3 (P13797)(Gly102-Asn375) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PLS3 recombinant protein

Background: PLS3, also known as plastin 3, belongs to the plastin family. Members of this family are actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. There are two ubiquitous plastin isoforms in humans: L and T. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). PLS3 contains 2 actin-binding domains, 4 CH (calponin-homology) domains and 2 EF-hand domains. It is expressed in a variety of organs, including muscle, brain, uterus and esophagus.

References

Lin CS. et al., 1993, J Biol Chem 268 (4): 2781-92.Goldstein D. et al., 1985, Cancer Res. 45 (2): 5643-7.Arpin M. et al., 1995, J Cell Biol. 127 (2): 1995-2008.

NCBI and Uniprot Product Information

• NCBI GI #: 226694201
• NCBI GeneID: 5358
• UniProt Accession #: P13797; Q86YI6; A8K579; B1AQ09; B4DGB4; B7Z6M1
• Molecular Weight: 630
• NCBI Official Full Name: Plastin-3
• NCBI Official Synonym Full Names: plastin 3
• NCBI Official Symbol: PLS3
• NCBI Official Synonym Symbols: BMND18; T-plastin
• NCBI Protein Information: plastin-3; T fimbrin; T plastin
• UniProt Protein Name: Plastin-3
• Protein Family: Plastin
• UniProt Gene Name: PLS3
• UniProt Entry Name: PLST_HUMAN

NCBI Description

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. Plastin 1 (otherwise known as Fimbrin) is a third distinct plastin isoform which is specifically expressed at high levels in the small intestine. The L isoform is expressed only in hemopoietic cell lineages, while the T isoform has been found in all other normal cells of solid tissues that have replicative potential (fibroblasts, endothelial cells, epithelial cells, melanocytes, etc.). The C-terminal 570 amino acids of the T-plastin and L-plastin proteins are 83% identical. It contains a potential calcium-binding site near the N terminus. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Feb 2010]

Uniprot Description

PLS3: Actin-bundling protein found in intestinal microvilli, hair cell stereocilia, and fibroblast filopodia.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: Xq23

Cellular Component: cytoplasm

Molecular Function: calcium ion binding; actin binding

Disease: Bone Mineral Density Quantitative Trait Locus 18