Calsequestrin 1 Recombinant Protein | CASQ1 recombinant protein

Calsequestrin 1 Protein, Human, Recombinant

Cat. Num. AAA8121541

• Purity: >80% as determined by SDS-PAGE
• Synonyms: Calsequestrin 1; Calsequestrin 1 Protein; Human; Recombinant; Human Calsequestrin-1/CASQ1 Protein; CASQ Protein; PDIB1 Protein; VMCQA Protein; calsequestrin 1 (fast-twitch; skeletal muscle); CASQ1 recombinant protein

• Host: E Coli
• Purity/Purification: >80% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 10% glycerol, pH7.5. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Gln35-Asp396

• Species: Human
• Predicted N Terminal: Met
• Tag: Native
• Protein Construction: A DNA sequence encoding the mature form of human CASQ1 (P31415)(Gln35-Asp396) was expressed with a N-terminal Met.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for CASQ1 recombinant protein

Background: Calsequestrin-1 is an isoform of calsequestrin. Calsequestrin is a calcium-binding protein of the sarcoplasmic reticulum. It helps hold calcium in the cisterna of the sarcoplasmic reticulum after a muscle contraction, even though the concentration of calcium in the sarcoplasmic reticulum is much higher than in the cytosol. Two forms of calsequestrin have been identified: Calsequestrin-2 and Calsequestrin-1. Calsequestrin-1 is found in fast skeletal muscle. The release of calsequestrin-bound calcium (through a calcium release channel) triggers muscle contraction. The active protein is not highly structured, more than 5% of it adopting a random coil conformation. When calcium binds there is a structural change whereby the alpha-helical content of the protein increases from 3 to 11%. Both forms of calsequestrin are phosphorylated by casein kinase 2, but the cardiac form is phosphorylated more rapidly and to a higher degree. Calsequestrin-1 is also secreted in the gut where it deprives bacteria of calcium ions.

References

Slupsky JR, et al. (1987) Characterization of cardiac calsequestrin. Biochemistry. 26(20): 6539-44. Cala SE, et al. (1991) Phosphorylation of cardiac and skeletal muscle calsequestrin isoforms by casein kinase II. Demonstration of a cluster of unique rapidly phosphorylated sites in cardiac calsequestrin. J Biol Chem. 266(1):391-8. Wang S, et al. (1998) Crystal structure of calsequestrin from rabbit skeletal muscle sarcoplasmic reticulum. Nat Struct Biol. 5(6):476-83.

NCBI and Uniprot Product Information

• UniProt Accession #: P31415
• Molecular Weight: The recombinant human CASQ1 consists of 363 amino acids and predicts a molecular mass of 41.8kDa. It migrates as an approximately 52kDa band in SDS-PAGE under reducing conditions.
• Protein Family: Calsequestrin