Pleckstrin Recombinant Protein | PLEK recombinant protein

Pleckstrin Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8121681

• Purity: >95% as determined by SDS-PAGE
• Synonyms: Pleckstrin; Pleckstrin Protein; Human; Recombinant (His Tag); Human Pleckstrin/PLEK/p47 Protein (His Tag); P47 Protein; pleckstrin; PLEK recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 50mM Tris, 10% Glycerol, pH8.0. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys350

• Species: Human
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the mature form of human PLEK (XP_004029394.1)(Met1-Lys350) was expressed with a polyhistide tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PLEK recombinant protein

Background: Pleckstrin is a protein found in platelets. Pleckstrin is the source of the name pleckstrin homology domain. Pleckstrin homology domain (PH domain) is a protein domain of approximately 12 amino acids that occurs in a wide range of proteins involved in intracellular signaling or as constituents of the cytoskeleton. This domain can bind Phosphatidylinositol lipids within biological membranes (such as Phosphatidylinositol (3,4,5)-trisphosphate and phosphatidylinositol (4,5)-bisphosphate), and proteins such as the betagamma-subunits of heterotrimeric G proteins, and protein kinase C. Through these interactions, PH domains play a role in recruiting proteins to different membranes, thus targeting them to appropriate cellular compartments or enabling them to interact with other components of the signal transduction pathways.

References

Ingley E, et al. (1994) Pleckstrin homology (PH) domains in signal transduction. J Cell Biochem. 56(4):436-43. Gibson TJ, et al. (1994) PH domain: the first anniversary. Trends Biochem Sci. 19(9):349-53. Haslam RJ, et al. (1993) Pleckstrin domain homology. Nature. 363(6427):309-10.

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant human PLEK consists of 365 amino acids and predicts a molecular mass of 41.9kDa. It migrates as an approximately 45kDa band in SDS-PAGE under reducing conditions.
• Protein Family: Pleckstrin