flagellin Recombinant Protein | FlaA recombinant protein

flagellin Protein, Listeria monocytogenes, Recombinant (His Tag)

Cat. Num. AAA8122145

• Purity: >95% as determined by SDS-PAGE
• Synonyms: flagellin; flagellin Protein; Listeria monocytogenes; Recombinant (His Tag); Listeria monocytogenes flagellin/flaA Protein (His Tag); flaA Protein; FlaA recombinant protein

• Host: E Coli
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ser287

• Species: Listeria monocytogenes
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the flaA (YP_013330.1)(Met1-Ser287) was expressed with a N-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for FlaA recombinant protein

Background: The role of flagella and motility in the attachment of the foodborne pathogen Listeria monocytogenes to various surfaces is mixed with some systems requiring flagella for an interaction and others needing only motility for cells to get to the surface. In nature this bacterium is a saprophyte and contaminated produce is an avenue for infection. Previous studies have documented the ability of this organism to attach to and colonize plant tissue. Motility mutants were generated in three wild type strains of L. monocytogenes by deleting either FlaA, the gene encoding flagellin, or motAB, genes encoding part of the flagellar motor, and tested for both the ability to colonize sprouts and for the fitness of that colonization. The motAB mutants were not affected in the colonization of alfalfa, radish, and broccoli sprouts; however, some of the FlaA mutants showed reduced colonization ability. The best colonizing wild type strain was reduced in colonization on all three sprout types as a result of a FlaA deletion. A mutant in another background was only affected on alfalfa. The third, a poor alfalfa colonizer was not affected in colonization ability by any of the deletions. Fitness of colonization was measured in experiments of competition between mixtures of mutant and parent strains on sprouts. Here the FlaA and motAB mutants of the three strain backgrounds were impaired in fitness of colonization of alfalfa and radish sprouts, and one strain background showed reduced fitness of both mutant types on broccoli sprouts. Together these data indicate a role for flagella for some strains to physically colonize some plants, while the fitness of that colonization is positively affected by motility in almost all cases.

References

Gorski L. et al., 2009, PLoS ONE. 4 (4): E5142. Tresse O. et al., 2009, Can J Microbiol. 55: 189-96. Schirm M. et al., 2004, J Bacteriol. 186: 6721-7.

NCBI and Uniprot Product Information

• Molecular Weight: The recombinant flaA consists of 302 amino acids and has a predicted molecular mass of 32.3kDa. It migrates as an approximately 33-37kDa band in SDS-PAGE under reducing conditions.
• Protein Family: Flagellin