Mouse interleukin 17F ELISA Kit | IL17F elisa kit

Mouse Interleukin-17F, IL17F ELISA Kit

Cat. Num. AAA920383

• Gene Names: Il17f; C87042; IL-17F
• Reactivity: Mouse
• Synonyms: interleukin 17F; Mouse Interleukin-17F; IL17F ELISA Kit; Mouse Interleukin-17F (IL17F) ELISA kit; IL-17F; ML-1; ML1; cytokine ML-1; IL17F elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse IL17F. No significant cross-reactivity or interference between mouse IL17F and analogues was observed.
• Sequence Length: 161

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 3.12 pg/ml -200 pg/ml.
• Sensitivity: The minimum detectable dose of mouse IL17F is typically less than 0.78 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL17F elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL17F has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL17F present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL17F is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL17F bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 119360346
• NCBI GeneID: 257630
• NCBI Accession #: NP_665855.2
• NCBI GenBank Nucleotide #: NM_145856.2
• UniProt Accession #: Q7TNI7; Q8K4C3
• Molecular Weight: 17,941 Da
• NCBI Official Full Name: interleukin-17F
• NCBI Official Synonym Full Names: interleukin 17F
• NCBI Official Symbol: Il17f
• NCBI Official Synonym Symbols: C87042; IL-17F
• NCBI Protein Information: interleukin-17F
• UniProt Protein Name: Interleukin-17F
• Protein Family: Interleukin
• UniProt Gene Name: Il17f
• UniProt Synonym Gene Names: IL-17F
• UniProt Entry Name: IL17F_MOUSE

Uniprot Description

IL17F: Stimulates the production of other cytokines such as IL- 6, IL-8 and granulocyte colony-stimulating factor, and can regulate cartilage matrix turnover. Stimulates PBMC and T-cell proliferation. Inhibits angiogenesis. Defects in IL17F are the cause of familial candidiasis type 6 (CANDF6). CANDF6 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans. Belongs to the IL-17 family.

Protein type: Cytokine; Secreted, signal peptide; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: protein homodimerization activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine binding; cytokine activity

Biological Process: proteoglycan metabolic process; negative regulation of angiogenesis; regulation of interleukin-8 biosynthetic process; regulation of transforming growth factor beta receptor signaling pathway; cytokine biosynthetic process; regulation of interleukin-2 biosynthetic process; cartilage development; lymphotoxin A biosynthetic process; regulation of granulocyte macrophage colony-stimulating factor biosynthetic process; regulation of interleukin-6 biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; inflammatory response